Recombinant Human Amphiphysin (AMPH)

Baculovirus
Mammalian cell

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

AMPH

Uniprot NO.

P49418

Alternative Names

Amp; AMPH 1; AMPH; AMPH_HUMAN; AMPH1; Amphiphysin (Stiff-Man syndrome with breast cancer 128kD autoantigen); Amphiphysin (Stiff-Man syndrome with breast cancer 128kDa autoantigen); Amphiphysin; Amphiphysin I; CG8604; Damp; dAmph; Dmel\CG8604; HGNC:471; MGC49429; Stiff man syndrome with breast cancer 128kD autoantigen; Stiff man syndrome with breast cancer 128kDa autoantigen

Species

Homo sapiens (Human)

Source

Yeast

Expression Region

1-695

Target Protein Sequence

MADIKTGIFA KNVQKRLNRA QEKVLQKLGK ADETKDEQFE EYVQNFKRQE AEGTRLQREL RGYLAAIKGM QEASMKLTES LHEVYEPDWY GREDVKMVGE KCDVLWEDFH QKLVDGSLLT LDTYLGQFPD IKNRIAKRSR KLVDYDSARH HLEALQSSKR KDESRISKAE EEFQKAQKVF EEFNVDLQEE LPSLWSRRVG FYVNTFKNVS SLEAKFHKEI AVLCHKLYEV MTKLGDQHAD KAFTIQGAPS DSGPLRIAKT PSPPEEPSPL PSPTASPNHT LAPASPAPAR PRSPSQTRKG PPVPPLPKVT PTKELQQENI ISFFEDNFVP EISVTTPSQN EVPEVKKEET LLDLDFDPFK PEVTPAGSAG VTHSPMSQTL PWDLWTTSTD LVQPASGGSF NGFTQPQDTS LFTMQTDQSM ICNLAESEQA PPTEPKAEEP LAAVTPAVGL DLGMDTRAEE PVEEAVIIPG ADADAAVGTL VSAAEGAPGE EAEAEKATVP AGEGVSLEEA KIGTETTEGA ESAQPEAEEL EATVPQEKVI PSVVIEPASN HEEEGENEIT IGAEPKETTE DAAPPGPTSE TPELATEQKP IQDPQPTPSA PAMGAADQLA SAREASQELP PGFLYKVETL HDFEAANSDE LTLQRGDVVL VVPSDSEADQ DAGWLVGVKE SDWLQYRDLA TYKGLFPENF TRRLD
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Full length protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton.

Gene References into Functions

The data show that the dynamin-amphiphysin helices are rearranged to form clusters upon GTP hydrolysis and membrane constriction occurs at protein-uncoated regions flanking the clusters. PMID:29357276
We show that AMPH-1/BIN1 binds to nesprin and actin, as well as to the microtubule-binding protein CLIP170 in both species. We propose that BIN1 has a direct and evolutionarily conserved role in nuclear positioning, altered in myopathies. PMID:26506308
It is a nerve protein and its autoantibody causes paraneoplastic neurological syndromes. PMID:25087559
AMPH1 protein level is reduced in transgenic mouse brain proteins. in the brainstem of nontransgenic and terminally ill JNPL3 transgenic mice. PMID:22975846
We propose that antibody-mediated amphiphysin deficiency may account for anxiety behavior in stiff person syndrome PMID:22331304
structure-based approach to prediction of peptide-binding behavior of SH3 domain of amphiphysin-1: statistical modeling PMID:19669081
investigation of binding affinities of decapeptide ligands with amphiphysin SH3 domain using QSAR models PMID:20214647
Female patient with amphiphysin autoantibodies and siff leg syndrome due to small cell cancer of the lung. PMID:16671079
Amphiphysin I (Amph I) is a minibrain kinase/dual-specificity tyrosine phosphorylation-regulated kinase substrate. Amph I phosphorylation changes the recruitment of endophilin at the endocytic sites. PMID:16733250
This study demonstrates that the analysis of the molecular interaction energy components between peptides and the SH3 domain can successfully characterize the binding interface. PMID:18206907
Analyses of Gaussian distribution models showed that diversified properties contribute to the interactions between the SH3 domain and of amphiphysin and peptides. PMID:18814309