Abbreviation
Recombinant Human CD38 protein, partial (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Greater than 95% as determined by SEC-HPLC.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA.Immobilized Human CD38 at 2 μg/mL can bind Anti-CD38 recombinant antibody(CSB-RA004929MA2HU). The EC50 is 20.07-28.73 ng/mL.
Alternative Names
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; EC:3.2.2.-; EC:3.2.2.6;2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-cyclic-ADP-ribose transferase; ADP-ribosyl cyclase 1 (ADPRC 1); Cyclic ADP-ribose hydrolase 1 (cADPR hydrolase 1); T10; CD38
Species
Homo sapiens (Human)
Expression Region
43-300aa
Target Protein Sequence
VPRWRQQWSGPGTTKRFPETVLARCVKYTEIHPEMRHVDCQSVWDAFKGAFISKHPCNITEEDYQPLMKLGTQTVPCNKILLWSRIKDLAHQFTQVQRDMFTLEDTLLGYLADDLTWCGEFNTSKINYQSCPDWRKDCSNNPVSVFWKTVSRRFAEAACDVVHVMLNGSRSKIFDKNSTFGSVEVHNLQPEKVQTLEAWVIHGGREDSRDLCQDPTIKELESIISKRNIQFSCKNIYRPDKFLQCVKNPEDSSCTSEI
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
C-terminal 6xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA.Immobilized Human CD38 at 2 μg/ml can bind Anti-CD38 recombinant antibody(CSB-RA004929MA2HU). The EC50 is 20.07-28.73 ng/mL.
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The purity of CD38 was greater than 95% as determined by SEC-HPLC
Description
CD38 is a multifunctional ectoenzyme central to NAD⁺ metabolism and a validated therapeutic target in hematologic malignancies, making reliable recombinant forms essential for mechanistic and drug discovery research. This partial human CD38 (residues 43–300), expressed in yeast with a C-terminal 6×His tag positioned away from the catalytic domain, retains confirmed binding activity with an EC₅₀ of 20.07–28.73 ng/mL against an anti-CD38 recombinant antibody in functional ELISA, supporting its use as a positive control in enzyme-linked assays and as a target for inhibitor screening and IC₅₀ determination. Purity exceeding 95% by SDS-PAGE and endotoxin levels below 1.0 EU/μg satisfy the stringent criteria typical for accurate kinetic parameter analysis (Km, Vmax, kcat), substrate specificity profiling, and drug candidate evaluation studies. These attributes also provide a suitable basis for structural investigations such as crystallography or cryo-EM where homogeneous, high-purity protein is critical.
