Home
Product
Protein
Recombinant Proteins
Recombinant Escherichia coli GTP pyrophosphokinase (relA)

Recombinant Escherichia coli GTP pyrophosphokinase (relA)

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

relA

Uniprot NO.

P0AG20

Species

Escherichia coli (strain K12)

Source

Yeast

Expression Region

1-744

Target Protein Sequence

MVAVRSAHIN KAGEFDPEKW IASLGITSQK SCECLAETWA YCLQQTQGHP DASLLLWRGV EMVEILSTLS MDIDTLRAAL LFPLADANVV SEDVLRESVG KSVVNLIHGV RDMAAIRQLK ATHTDSVSSE QVDNVRRMLL AMVDDFRCVV IKLAERIAHL REVKDAPEDE RVLAAKECTN IYAPLANRLG IGQLKWELED YCFRYLHPTE YKRIAKLLHE RRLDREHYIE EFVGHLRAEM KAEGVKAEVY GRPKHIYSIW RKMQKKNLAF DELFDVRAVR IVAERLQDCY AALGIVHTHY RHLPDEFDDY VANPKPNGYQ SIHTVVLGPG GKTVEIQIRT KQMHEDAELG VAAHWKYKEG AAAGGARSGH EDRIAWLRKL IAWQEEMADS GEMLDEVRSQ VFDDRVYVFT PKGDVVDLPA GSTPLDFAYH IHSDVGHRCI GAKIGGRIVP FTYQLQMGDQ IEIITQKQPN PSRDWLNPNL GYVTTSRGRS KIHAWFRKQD RDKNILAGRQ ILDDELEHLG ISLKEAEKHL LPRYNFNDVD ELLAAIGGGD IRLNQMVNFL QSQFNKPSAE EQDAAALKQL QQKSYTPQNR SKDNGRVVVE GVGNLMHHIA RCCQPIPGDE IVGFITQGRG ISVHRADCEQ LAELRSHAPE RIVDAVWGES YSAGYSLVVR VVANDRSGLL RDITTILANE KVNVLGVASR SDTKQQLATI DMTIEIYNLQ VLGRVLGKLN QVPDVIDARR LHGS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

full length protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Customer Reviews and Q&A

■ Customer Reviews

Submit a Review here

Target Background

Function(From Uniprot)

In eubacteria ppGpp (guanosine 3'-diphosphate 5'-diphosphate) is a mediator of the stringent response which coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which is then hydrolyzed to form ppGpp. The second messengers ppGpp and c-di-GMP together control biofilm formation in response to translational stress; ppGpp represses biofilm formation while c-di-GMP induces it. ppGpp activates transcription of CsrA-antagonistic small RNAs CsrB and CsrC, which downregulate CsrA's action on translation during the stringent response.

Gene References into Functions

The boomerang-shaped RelA with a wingspan of more than 100 A wraps around the A/R (30S A-site/RelA-bound) tRNA. PMID:27434674
We propose that the open conformation of RelA on the ribosome relieves the autoinhibitory effect of the CTD on the NTD, thus leading to stimulation of (p)ppGpp synthesis by RelA. PMID:27226493
Here we review the role of (p)ppGpp in bacterial cell cycle regulation PMID:27240988
Amino acid starvation induces net binding of RelA to 70S ribosomes. PMID:26480956
Mutations in relA, but not necessarily inactivating mutations, can be selected to compensate total SpoT function(s) loss in Escherichia coli. PMID:25188023
Transcription of relA, a key gene responsible for the synthesis of ppGpp, is activated by NtrC during nitrogen starvation. PMID:24947454
ppGpp dramatically increases the turnover rate of its own ribosome-dependent synthesis by RelA PMID:22814757
RelA protein stimulates the activity of RyhB small RNA by acting on RNA-binding protein Hfq PMID:22393021
Data show that RelA is on the ribosome under nonstarved conditions. PMID:21730169
6S RNA regulates transcription of relA encodes a ppGpp synthase. PMID:20829285
suggestion that stringent factor formed a complex with tRNA in solution that had higher affinity for the ribosomal A-site PMID:15670150
Six different L11 point and deletion-mutants have been constructed to determine which regions of L11 are necessary for the activation of stringent factor. PMID:17095013
the omega subunit of RNA polymerase is involved in regulation of the relA gene PMID:17233676
The interaction between (p)ppGpp synthases and acyl carrier protein restricted to bacteria containing the RelA and SpoT proteins. PMID:18996989
RelA has a previously unrecognized role in ribosome function. PMID:19168615