relA Antibody

Code: CSB-PA360357XA01ENV

Rate （N/A）
Review （0）

Size:

0.1ml

0.1ml1ml

US$Enquire

Quantity:

Species Reactivity: Escherichia coli (strain K12)

Raised in: Rabbit

Application: ELISA, WB (ensure identification of antigen)

Send an Inquiry

Start an on-line Chat

Online ordering is currently available for U.S. customers only. For orders outside the U.S., please kindly submit an inquiry or start a chat with us.

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot NO.

P0AG20

Target Names

relA

Alternative Names

relA antibody; b2784 antibody; JW2755 antibody; GTP pyrophosphokinase antibody; EC 2.7.6.5; antibody; p)ppGpp synthase antibody; ATP:GTP 3'-pyrophosphotransferase antibody; ppGpp synthase I antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) relA protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Tested Applications

ELISA, WB (ensure identification of antigen)

Lead Time

Made-to-order (14-16 weeks)
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Datasheet & COA

Antibody FAQs

Customer Reviews and Q&A

■ Customer Reviews

Submit a Review here

Target Background

Function(From Uniprot)

In eubacteria ppGpp (guanosine 3'-diphosphate 5'-diphosphate) is a mediator of the stringent response which coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which is then hydrolyzed to form ppGpp. The second messengers ppGpp and c-di-GMP together control biofilm formation in response to translational stress; ppGpp represses biofilm formation while c-di-GMP induces it. ppGpp activates transcription of CsrA-antagonistic small RNAs CsrB and CsrC, which downregulate CsrA's action on translation during the stringent response.

Gene References into Functions

The boomerang-shaped RelA with a wingspan of more than 100 A wraps around the A/R (30S A-site/RelA-bound) tRNA. PMID:27434674
We propose that the open conformation of RelA on the ribosome relieves the autoinhibitory effect of the CTD on the NTD, thus leading to stimulation of (p)ppGpp synthesis by RelA. PMID:27226493
Here we review the role of (p)ppGpp in bacterial cell cycle regulation PMID:27240988
Amino acid starvation induces net binding of RelA to 70S ribosomes. PMID:26480956
Mutations in relA, but not necessarily inactivating mutations, can be selected to compensate total SpoT function(s) loss in Escherichia coli. PMID:25188023
Transcription of relA, a key gene responsible for the synthesis of ppGpp, is activated by NtrC during nitrogen starvation. PMID:24947454
ppGpp dramatically increases the turnover rate of its own ribosome-dependent synthesis by RelA PMID:22814757
RelA protein stimulates the activity of RyhB small RNA by acting on RNA-binding protein Hfq PMID:22393021
Data show that RelA is on the ribosome under nonstarved conditions. PMID:21730169
6S RNA regulates transcription of relA encodes a ppGpp synthase. PMID:20829285
suggestion that stringent factor formed a complex with tRNA in solution that had higher affinity for the ribosomal A-site PMID:15670150
Six different L11 point and deletion-mutants have been constructed to determine which regions of L11 are necessary for the activation of stringent factor. PMID:17095013
the omega subunit of RNA polymerase is involved in regulation of the relA gene PMID:17233676
The interaction between (p)ppGpp synthases and acyl carrier protein restricted to bacteria containing the RelA and SpoT proteins. PMID:18996989
RelA has a previously unrecognized role in ribosome function. PMID:19168615