Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
BHRF1Apoptosis regulator BHRF1; Early antigen protein R; EA-R; Nuclear antigen
Species
Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
Expression Region
1-142aa
Target Protein Sequence
MAYSTREILLALCIRDSRVHGNGTLHPVLELAARETPLRLSPEDTVVLRYHVLLEEIIERNSETFTETWNRFITHTEHVDLDFNSVFLEIFHRGDPSLGRALAWMAWCMHACRTLCCNQSTPYYVVDLSVRGMLEASEGLDG
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
N-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Description
The expression region of this recombinant Epstein-Barr virus BHRF1 covers amino acids 1-142. The expected molecular weight for the BHRF1 protein is calculated to be 19.8 kDa. This protein is generated in a e.coli-based system. The N-terminal 10xHis tag was smoothly integrated into the coding gene of BHRF1, which enables a simple process of detecting and purifying the BHRF1 recombinant protein in the following steps.
The Epstein-Barr virus (EBV) apoptosis regulator BHRF1 is a homolog of the cellular Bcl-2 protein, which plays a crucial role in regulating apoptosis and inhibiting the intrinsic apoptotic pathway. Similarly, BHRF1 functions as an anti-apoptotic protein in EBV-infected cells. By interacting with pro-apoptotic factors and preventing mitochondrial outer membrane permeabilization, BHRF1 helps the virus evade the host immune response and establish persistent infections. It is particularly involved in blocking the apoptotic response induced by various cellular stress signals, contributing to the survival of infected cells. Understanding the mechanisms employed by BHRF1 in modulating apoptosis is crucial for elucidating the pathogenesis of EBV-associated diseases and may have implications for the development of antiviral strategies.
