Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
IFNG; Interferon gamma; IFN-gamma
Species
Gallus gallus (Chicken)
Expression Region
20-164aa
Target Protein Sequence
HTASSLNLVQLQDDIDKLKADFNSSHSDVADGGPIIVEKLKNWTERNEKRIILSQIVSMYLEMLENTDKSKPHIKHISEELYTLKNNLPDGVKKVKDIMDLAKLPMNDLRIQRKAANELFSILQKLVDPPSFKRKRSQSQRRCNC
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal GST-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The Recombinant Chicken IFNG protein is a protein encoded by recombinant DNA that was cloned in an expression vector that supported the expression of IFNG gene. This recombinant IFNG protein was expressed in the host. The expression region is 20-164aa of the Chicken IFNG. In the production, the expression vector contains N-terminal GST tag. Every production step was performed with a strict QC system. The purity of this protein is 90%+ determined by SDS-PAGE.
interferon-gamma/Interferon γ coded by the IFNG gene is among the common cytokines known to affect immune related disorders. Blockade of PD1/PD-L1 activates T cells accompanied by interferon- gamma (IFN-g) secretion, which has been proposed to mediate both pro- and anti-tumoral activities. IFN-g mediates adaptive resistance through PD-L1 dependent and independent mechanisms, which is becoming a key issue to broaden cancer immunotherapy. Other research demonstrates that liver type 1 innate lymphoid cells develop locally via an interferon-γ–dependent loop. Specifically, deficiency in the gene encoding interferon-γ or one of its receptors (Ifngr1) selectively reduced the frequency and number of ILC1s and not cNK cells in the liver. Delivery of a plasmid containing the interferon-γ (IFN-γ) cDNA to IFN-g-deficient mice via hydrodynamic tail-vein injection selectively increased the frequency and number of liver’s ILC1s but not of liver cNK cells. Moreover, IFN-γ signaling promoted the expansion and differentiation of LSM cells but not of ILC1s, supporting a model in which IFN-γ acts on these local progenitors to promote liver ILC1 development. The findings advance researchers’ knowledge of the importance of extramedullary hematopoiesis to cells of lymphoid origin.
