Chicken Interferon γ,IFN-γ ELISA Kit

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Name: Chicken Interferon γ,IFN-γ ELISA Kit
Brand: CUSABIO
SKU: CSB-E08550Ch

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Code: CSB-E08550Ch

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Citation （38）

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 3.125 pg/mL-800 pg/mL

Sensitivity: 3.125 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Target Name

interferon, gamma

Alternative Names

IFNG ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit

Abbreviation

IFNG

Uniprot NO.

P49708

Species

Gallus gallus (Chicken)

Sample Type

serum, plasma, tissue homogenates

Detection Range

3.125 pg/mL-800 pg/mL

Sensitivity

3.125 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Competitive

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of chicken IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	91-100
1:2	Average %	92
	Range %	88-97
1:4	Average %	102
	Range %	98-106
1:8	Average %	94
	Range %	90-98

Recovery

The recovery of chicken IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	93-101
EDTA plasma (n=4)	92	88-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
800	0.178	0.182	0.180
200	0.245	0.233	0.239
50	0.457	0.482	0.470
12.5	0.848	0.817	0.833
3.125	1.396	1.364	1.380
0	2.237	2.242	2.240

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Chicken Interferon γ,IFN-γ ELISA Kit

Description

This Chicken IFNG ELISA Kit was designed for the quantitative measurement of Chicken IFNG protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 3.125 pg/mL-800 pg/mL and the sensitivity is 3.125 pg/mL.

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■ Q&A

Do you know if the chicken IFN-gamma kit shows cross-reactivity with other species?

There is no cross-reactivity with human, rat, and mouse recombinant proteins.

Lactobacillus acidophilus as a live vehicle for oral immunization against chicken anemia virus Hassan Moeini et al,/,2010
Bursopentine as a Novel Immunoadjuvant Enhances both Humoral and Cell-Mediated Immune Responses to Inactivated H9N2 Avian Influenza Virus in Chickens /,/,2011
Development of a DNA vaccine against chicken anemia virus by using a bicistronic vector expressing VP1 and VP2 proteins of CAV /,/,2011
Improving the potency of DNA vaccine against Chicken Anemia Virus (CAV) by fusing VP1 protein of CAV toMarek's Disease Virus (MDV) Type-1 VP22 protein Hassan Moeini et al,/,2011
Omega-3 polyunsaturated fatty acids enrichment alters performance and immune response in infectious bursal disease challenged broilers Elham Maroufyan et al,/,2012
Expression analysis of programmed death ligand 2 in tumors caused by the avian oncovirusMarek's disease virus Matsuyama-Kato A et al,/,2014
Eimeria maxima microneme protein 2 delivered as DNA vaccine and recombinant protein induces immunity against experimental homogenous challenge Huang J. et al,/,2015
Immune protection of Microneme 7 (EmMIC7) against Eimeria maxima challenge in chickens Huang J. et al,/,2015
Immune responses to oral vaccination with Salmonella-delivered avian pathogenic Escherichia coli antigens and protective efficacy against colibacillosis. Lee JH.et al,/,2015
Glycated serum albumin stimulates expression of endothelial cell specific molecule-1 in human umbilical vein endothelial cells: Implication in diabetes mediated endothelial dysfunction Nirala BK. et al,/,2015
Influenza Inactive Virus Vaccine with the Fusion Peptide (rTa1- BP5) Enhances Protection Against Influenza Through Humoral and Cell-Mediated Immunity Chen Wang.et al,/,2016
Effect of Antibiotic Growth Promoters on Anti-oxidative and Anti-inflammatory Activities in Broiler Chickens Kabploy K.et al,/,2016
Immunization with avian metapneumovirus harboring chicken Fc induces higher immune responses. Paudel S.et al,/,2016
The Molecular Characterization and Immunity Identification of Microneme 3 of Eimeria acervulina. Zhang Z.et al,/,2016
Heat stressDecreases expression of the cytokines, avian B-defensins 4 and 6 and Toll-like receptor 2 in broiler chickens infected with Salmonella Enteritidis W.M. Quinteiro-Filho.et al,/,2017
Validation of immunomodulatory effects of lipopolysaccharide through expression profiling of Th1 and Th2 biased genes in Newcastle disease virus vaccinated indigenous chicken Rabia Bhardwaj.et al,/,2018
Molecular characterisation and the protective immunity evaluation of Eimeria maxima surface antigen gene Tingqi Liu.et al,/,2018
Gene cloning, expression and immune adjuvant properties of the recombinant fusion peptide Ta1-BLP on avian influenza inactivate virus vaccine CongZhang.et al,/,2018
The molecular characterization and protective efficacy of microneme 3 of Eimeria mitis in chickens XinmeiHuang.et al,/,2018
Dietary Supplementation of Purified Amino Acid Derived from Animal Blood on Immune Response and Growth Performance of Broiler Chicken T. G. Wandita.et al,/,2018
Grape Seed Proanthocyanidin Extract Alleviates AflatoxinB1-Induced Immunotoxicity and Oxidative Stress via Modulation of NF-κB and Nrf2 Signaling Pathways in Broilers Shahid Ali Rajput, et al,/,2019
Effects of Supplementing Laying Hens with Purified Amino Acid Prepared from Animal Blood N. Joshi, et al,/,2019
The effects and combinational effects of Bacillus subtilis and montmorillonite supplementation on performance, egg quality, oxidation status, and immune response in laying hens J.F.Chen, et al,/,2019
Lack of Toxic Interaction Between Fusariotoxins in Broiler Chickens Fed throughout Their Life at the Highest Level Tolerated in the European Union Metayer JP, et al,/,2019
Immunogenic potential and protective efficacy of a sptP deletion mutant of Salmonella Enteritidis as a live vaccine for chickens against a lethal challenge Yaxin Guo, et al,/,2019
Effect of Acute Heat Stress on the mRNA Levels of Cytokines in Broiler Chickens Subjected to Embryonic Thermal Manipulation Saleh KMM, et al,/,2019
A Novel Adjuvant “Sublancin” Enhances Immune Response in Specific Pathogen-Free Broiler Chickens Inoculated with Newcastle Disease Vaccine Liu Y, et al,/,2019
In vivo immunoprotective comparison between recombinant protein and DNA vaccine of Eimeria tenella surface antigen 4 P Zhao,/,2020
Effects of different levels of Hermetia illucens larvae meal on performance, egg quality, yolk fatty acid composition and oxidative status of laying hens Xin Liu,/,2021
Evaluation of immunoprotective effects of recombinant protein and DNA vaccine based on Eimeria tenella surface antigen 16 and 22 in vivo Rui Fang,/,2021
Synergistic Immunosuppression of Avian Leukosis Virus Subgroup J and Infectious Bursal Disease Virus Is Responsible for Enhanced Pathogenicity W Chen,/,2022
Comparative Analysis of the Liver Transcriptome of Beijing You Chickens and Guang Ming Broilers under Salmonella enterica Serovar Typhimurium Infection Z Wang,/,2022
Impact of stocking density and dietary nano-zinc supplementation on stress indicators, immunity, and DNA damage in broiler chickens Ö Sevim,/,2023
Effect of Two Particle Sizes of Nano Zinc Oxide on Growth Performance, Immune Function, Digestive Tract Morphology, and Intestinal Microbiota Composition in Broilers J Qu,/,2023
Investigation of the Potential of Heterophil/Lymphocyte Ratio as a Biomarker to Predict Colonization Resistance and Inflammatory Response to Salmonella enteritidis Infection in Chicken M Thiam,/,2023
The inhibitory effect of Hypericum japonicum on H9N2 avian influenza virus H Hu, J Li, S Zheng, K Zhao, Y Xia, X Wei, M Han,/,2024
Modulation of Intestinal Health and Metabolism by Dietary Portulaca oleracea L. Extract Enhances Growth Performance, Immune Function, and Meat Quality in Wenchang chickens Y Zhang, Y Yang, Y Li, Y Sun, X Han, H Liu, X Zhao,/,2025
Spleen transcriptomics reveals breed-specific mechanisms of Salmonella resistance under heat stress in chickens Y Wang, Q Wang, Z Wang, X Li, MS Elsharkawy, Y Lu,/,2025

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

Gene References into Functions

Knockdown of IFN-gamma did not seem to fully abrogate vaccine-induced protection against Marek's disease in chickens. PMID:25673902
Vitiligo in Smyth line chickens appears to be a Th1-polarized autoimmune disease, whereby IFN-gamma expression is strongly associated with parallel increases in IL-10 and IL-21, particularly during the early and active stages of vitiligo. PMID:22113479
The dose-response effects of supplemental dietary chormium on interferon-gamma expression in response to Newcastle disease virus vaccine in broiler chickens is reported. PMID:17888472
The pivotal role of chicken interferon-gamma in the pathogenesis and immunosuppression of infectious bursal disease virus infection in in vivo, ex vivo and in vitro experiments are reported. PMID:17899460
that in vitro stimulation with IBV resulted in ChIFN-gamma production in splenocytes of both infected birds and uninfected birds PMID:19070879