| Application | Recommended Dilution |
|---|---|
| WB | 1:500-1:5000 |
| IF | 1:50-1:200 |
Western Blot
Positive WB detected in: Hela whole cell lysate, LO2 whole cell lysate, A549 whole cell lysate, PC-3 whole cell lysate
All lanes: VN1R1 antibody at 3.3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
Immunofluorescence staining of Hela cells with CSB-PA861973LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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