UMODL1 Antibody

CSB-PA025617LA01HU

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US$83

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Species Reactivity: Human, Mouse

Raised in: Rabbit

Application: ELISA, WB, IHC, IF

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Target Background

Product Details

Uniprot NO.

Q5DID0

Target Names

UMODL1

Alternative Names

D17Ertd488e antibody; Olfactorin antibody; OTTHUMP00000109312 antibody; OTTHUMP00000109313 antibody; RGD1566039 antibody; UMODL1 antibody; UROL1_HUMAN antibody; Uromodulin-like 1 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Uromodulin-like 1 protein (537-654AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Protocols

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: NIH/3T3 whole cell lysate, MCF-7 whole cell lysate
All lanes: UMODL1 antibody at 5.1µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 145, 157, 137, 150 kDa
Observed band size: 145, 157 kDa
IHC image of CSB-PA025617LA01HU diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA025617LA01HU diluted at 1:300 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of MCF-7 cells with CSB-PA025617LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Description

This antibody reacts against the human and mouse UMOLD1 protein. It was raised in rabbit using the partial human UMOLD1 protein as the immunogen. The immunogen region corresponds to the 537-654aa of Recombinant Human UMOLD1 that is a section of the second EGF-like domain. This antibody is a non-conjugate polyclonal IgG with a purity higher than 95% purified by protein G. The uses of this antibody were tested on ELISA, Western blot (WB), Immunohistochemistry (IHC), and Immunofluorescence (IF) applications. The target protein UMOLD1 participates in the response of gonadotropin-releasing hormone, regulates gene expression, and with that, the adipose tissue and ovarian follicle development. The domains of the protein are suggested to bind calcium, interact with proteins, carbohydrate side chains, and other types of substrates. Also, UMOLD1 could have an antiproteinase function, and the presence of the zona pellucida domain (ZP) suggests that the UMOLD1 could have fundamental roles in development, hearing, immunity, or cancer. Indeed, some evidence suggests that it plays a role in high myopia susceptibility.

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Target Background

Gene References into Functions

It was shown that common UMODL1 polymorphisms are unlikely to be important in the genetic susceptibility to high myopia in Han Chinese. PMID:22857148
Data report the isolation of a novel gene, designated UMODL1, similar to uromodulin (UMOD)/Tamm-Horsfall glycoprotein, on human chromosome 21q22.3. PMID:15194491
result suggests that rs283971 is located in the frequent recombinant region on the UMODL1, and that this region might play a critical role in disease susceptibility to high myopia. PMID:18535602

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Subcellular Location

Cell membrane; Single-pass type I membrane protein.; [Isoform 3]: Cytoplasm.; [Isoform 4]: Cytoplasm.

Tissue Specificity

Isoform 4 is expressed at low level in kidney, testis and fetal thymus. Isoform 3 is expressed at low level in prostate, testis and fetal thymus.

Database Links

HGNC: 12560

UNIGENE: Hs.242520

KEGG: hsa:89766

OMIM: 613859

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