NMT2 Antibody

Code: CSB-PA015901DSR2HU

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20μl50μl100μl

US$83

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Species Reactivity: Human

Raised in: Rabbit

Application: ELISA, WB, IHC, IP

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Product Details
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Target Background

Product Details

Uniprot NO.

O60551

Target Names

NMT2

Alternative Names

EC 2.3.1.97 antibody; Glycylpeptide N tetradecanoyltransferase 2 antibody; Glycylpeptide N-tetradecanoyltransferase 2 antibody; Myristoyl CoA:protein N myristoyltransferase 2 antibody; Myristoyl-CoA:protein N-myristoyltransferase 2 antibody; N-myristoyltransferase 2 antibody; NMT 2 antibody; NMT2 antibody; NMT2_HUMAN antibody; Peptide N myristoyltransferase 2 antibody; Peptide N-myristoyltransferase 2 antibody; Type II N myristoyltransferase antibody; Type II N-myristoyltransferase antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Glycylpeptide N-tetradecanoyltransferase 2 protein (1-120AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB, IHC, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200
IP	1:200-1:2000

Protocols

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Immunoprecipitation (IP) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western blot
All lanes: NMT2 antibody at 4.99μg/ml
Lane 1: Human placenta tissue
Lane 2: K562 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA015901DSR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA015901DSR2HU at dilution of 1:100
Immunoprecipitating NMT2 in HepG2 whole cell lysate
Lane 1: Rabbit control IgG instead of (1μg) instead of CSB-PA015901DSR2HU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA015901DSR2HU (4μg) + HepG2 whole cell lysate (500μg)
Lane 3: HepG2 whole cell lysate (20μg)

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Target Background

Function(From Uniprot)

Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins.

Gene References into Functions

ligand binding properties of the NMT/ACBD6 complex can explain how the NMT reaction can proceed in the presence of the very abundant competitive substrate, C(16)-CoA. PMID:26621918
induction of NMT2 may play a central role in dioxin carcinogenicity. PMID:11731439
NMT1 and NMT2 have only partially overlapping functions; NMT1 is critical for tumor cell proliferation PMID:16123142
Involved in progression and regulation of telomerase PMID:16364039
Protein-protein interaction of NMTs revealed that m-calpain interacts with NMT1 while caspase-3 interacts with NMT2. PMID:16530191
Disruption of the gene for NMT2 by a t(8;10) translocation in a male with hypogonadism. PMID:17568424
Nef is preferentially myristoylated by NMT2, suggesting that selective inhibition of NMT2 may provide a novel means of blocking HIV virulence. PMID:18089753