ITGB6 Antibody

Code: CSB-PA011890LA01HU

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20μl50μl100μl

US$83

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Species Reactivity: Human, Mouse

Raised in: Rabbit

Application: ELISA, WB,IF

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Product Details
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Target Background

Product Details

Uniprot NO.

P18564

Target Names

ITGB6

Alternative Names

ITGB6; Integrin beta-6

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Integrin beta-6 protein (451-586AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB,IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:20-1:100

Protocols

ELISA Protocol Western Blotting(WB) Protocol Immunofluorescence (IF) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: NIH/3T3 whole cell lysate(30µg), A549 whole cell lysate(30µg), HT29 whole cell lysate(30µg),HEK293 whole cell lysate(30µg)
All lanes: ITGB6 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 86,75 kDa
Observed band size: 86 kDa
Exposure time: 10s
Immunofluorescence staining of A549 cells with CSB-PA011890LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Description

ITGB6 antibody CSB-PA011890LA01HU is a rabbit polyclonal IgG that binds to human and rat ITGB6. It was produced in a rabbit immunized with the recombinant human ITGB6 protein (451-586aa). This ITGB6 antibody has been selected for its ability to recognize ITGb6 in ELISA, WB, IHC, and IF. Its purity reaches up to 95% using protein G affinity chromatography.

ITGB6 is primarily expressed in epithelial cells, where it plays a critical role in regulating cell-ECM interactions and tissue remodeling. It has also been implicated in inflammation, wound healing, and cancer progression. Variations in ITGB6 protein have been associated with various pathological conditions, including fibrosis, cancer, and autoimmune diseases.

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Target Background

Function(From Uniprot)

Integrin alpha-V:beta-6 (ITGAV:ITGB6) is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalization of integrin alpha-V/beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion. ITGAV:ITGB6 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1. Integrin alpha-V:beta-6 (ITGAV:ITGB6) mediates R-G-D-dependent release of transforming growth factor beta-1 (TGF-beta-1) from regulatory Latency-associated peptide (LAP), thereby playing a key role in TGF-beta-1 activation.; (Microbial infection) Integrin ITGAV:ITGB6 acts as a receptor for Coxsackievirus A9 and Coxsackievirus B1.; (Microbial infection) Integrin ITGAV:ITGB6 acts as a receptor for Herpes simplex virus-1/HHV-1.

Gene References into Functions

these findings demonstrate that JunB and CBP-mediated histone hyperacetylation are responsible for TGF-beta1 induced ITGB6 transcription in oral squamous cell carcinoma (OSCC) cells, suggesting that epigenetic mechanisms are responsible for the active transcription expression of ITGB6 induced by TGF-beta1 in OSCC cells. PMID:29274289
Taken together, our results indicate that integrin beta6 promotes tumor invasiveness in a Rac1-dependent manner and is a potential biomarker for tumor metastasis. PMID:27440504
The increased regional and distant metastasis observed in HER2+ tumors with high levels of ITGB6 might be mediated by the canonical Rho-Rac pathway through increased expression of MMP9 and MMP15. PMID:27184932
Rare missense variant c.898G>A (p.(Glu300Lys)) in ITGB6 causes not only dentogingival anomalies but also intellectual disability and alopecia. PMID:26695873
Treatment of these cells with the dual-specificity tyrosine-kinase inhibitor lapatinib led to downregulation of epithelial-to-mesenchymal transition as indicated by lower levels of SNAI1 and SNAI2 transcripts, integrin AVB6, and matrix metalloproteinase 9 protein PMID:28349782
Integrin b6 markedly promoted the proliferation and invasion of pancreatic carcinoma cells and induced ETS1 phosphorylation in an ERK-dependent manner, leading to the upregulation of matrix metalloprotease-9, which is essential for b6-mediated invasiveness of pancreatic carcinoma cells PMID:26547582
AG/GG genotypes of ITGB6 rs4665162 gene were associated with a higher risk of radiation pneumonitis (RP) in patients with lung cancer receiving radiotherapy and thus may serve as a reliable predictor of RP. PMID:26449830
A novel homozygous ITGB6 mutation causing isolated amelogenesis imperfecta, and this advances the understanding of normal and pathologic enamel development. PMID:25431241
These data reveal a novel role for Elk1 regulating ITGB6 expression and highlight how dysregulation of Elk1 can contribute to human disease. PMID:26861876
Integrin beta6 expression correlated significantly with MMP-9 expression, and may be a valuable recurrence indicator for follicular thyroid carcinomas PMID:24844802
The results indicated that MMP-9-degraded Fibronectin can adjust constituents of the avb6 heterodimers at the early phase and significantly elevate amounts of b6 integrin subunits at a later period PMID:26134759
Results shows that ITGB6 promoter contains a functional TATA box and that STAT3 and C/EBPalpha are involved in its positive regulation in oral squamous cell carcinoma cells. PMID:25816241
targeting eIF4E in integrin ava6 expressing tumors can be a potential therapeutic strategy for patients with colon cancer. PMID:25982998
In conclusion NSCLC cell lines, positive for E-Cadherin,EpCAM and avb6 expression, activate normal fibroblasts through avbeta6/TGFbeta signalling in vitro, and influence both gene expression and response to therapeutic agents. PMID:24488011
The deficiency of avb6-related hyperpermeability in T84 monolayers could be compensated by adding exogenous avb6 to the culture. PMID:24677750
Expression of the alphavbeta6 integrin subunit seems to be of pathobiological and clinical relevance in patients with non-small cell lung cancer with brain metastases. PMID:25150423
ITGB6 loss-of-function mutations cause autosomal recessive amelogenesis imperfecta. PMID:24305999
Brain metastases ITGB6 expression exhibits considerable heterogeneity according to tumor origin. PMID:24294359
ADAM 10 is over expressed in oral squamous cell carcinoma and contributes to invasive behaviour through a functional association with alphavbeta6 integrin PMID:24055471
Data indicate that integrin beta6, CD46, tissue factor, and chromosome 14 open reading frame 1 (C14ORF1), were identified as overexpressed on pancreatic cancer cell lines. PMID:21934552
These data identify a novel interaction between Psor and beta6 and demonstrate that it is required for alphavbeta6-dependent invasion by carcinoma cells. PMID:21132011
Integrin beta6 plays a role in the compromised wound healing associated with the diabetic state. PMID:20854469
Fusion of epithelial cells by Epstein-Barr virus proteins is triggered by binding of viral glycoproteins gHgL to integrins alphavbeta6 or alphavbeta8. PMID:19920174
Integrin expression in colon cancer cells is regulated by the cytoplasmic domain of the beta6 integrin subunit PMID:11992542
Loss of integrin alpha(v)beta6-mediated TGF-beta activation causes Mmp12-dependent emphysema PMID:12634787
upon ligation of integrin beta6 with fibronectin, beta6 complexed with Fyn and activated it, activating a pathway leading to activation of the matrix metalloproteinase-3 gene, and promoting oral SCC cell proliferation and experimental metastasis in vivo PMID:12917446
beta(6) integrin is not normally expressed in adult native or transplanted kidneys but is commonly up-regulated in the distal tubule in disease. PMID:15458435
Constitutively activated STAT3 induces tumorigenesis and enhances cell motility of prostate epithelial cells through ITGB6. PMID:17438134