| Application | Recommended Dilution |
|---|---|
| WB | 1:500-1:5000 |
| IHC | 1:20-1:200 |
| IF | 1:50-1:200 |
Western Blot
Positive WB detected in: Jurkat whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate
All lanes: HLA-B antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
Immunohistochemistry of paraffin-embedded human spleen tissue using CSB-PA15089A0Rb at dilution of 1:100
Immunofluorescent analysis of HepG2 cells using CSB-PA15089A0Rb at dilution of 1:100
The product CSB-PA15089A0Rb is a polyclonal antibody against HLA-B. It was developed in the rabbit and used the recombinant human HLA-B protein (25-308aa) as the immunogen. Following the protein G affinity chromatography purification, this HLA-B polyclonal antibody got up to 95% purity.
This antibody is an unconjugated IgG. It has been validated to recognize the human HLA-B protein in the ELISA, WB, IHC, and IF applications. It may assist in investigating the HLA-B protein functions in environmental contexts.
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