FUT3 Antibody

CSB-PA009077LA01HU

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US$83

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Species Reactivity: Human, Rat

Raised in: Rabbit

Application: ELISA, WB, IHC

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Product Details
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Target Background

Product Details

Uniprot NO.

P21217

Target Names

FUT3

Alternative Names

FUT3; FT3B; LE; 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase FUT3; Alpha-3-fucosyltransferase FUT3; Blood group Lewis alpha-4-fucosyltransferase; Lewis FT; Fucosyltransferase 3; Fucosyltransferase III; FucT-III

Raised in

Rabbit

Species Reactivity

Human, Rat

Immunogen

Recombinant Human Galactoside 3(4)-L-fucosyltransferase protein (83-187AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:100-1:300

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Protocols

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol

Datasheet & COA

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Images

Western Blot
Positive WB detected in: Rat lung tissue
All lanes: FUT3 antibody at 5.3μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa
IHC image of CSB-PA009077LA01HU diluted at 1:300 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Description

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Target Background

Function

Catalyzes the transfer of L-fucose, from a guanosine diphosphate-beta-L-fucose, to both the subterminal N-acetyl glucosamine (GlcNAc) of type 1 chain (beta-D-Gal-(1->3)-beta-D-GlcNAc) glycolipids and oligosaccharides via an alpha(1,4) linkage, and the subterminal glucose (Glc) or GlcNAc of type 2 chain (beta-D-Gal-(1->4)-beta-D-GlcNAc) oligosaccharides via an alpha(1,3) linkage, independently of the presence of terminal alpha-L-fucosyl-(1,2) moieties on the terminal galactose of these acceptors and participates in the blood groups Lewis determination and expression of Lewis a (Le(a)), lewis b (Le(b)), Lewis x/SSEA-1 (Le(x)) and lewis y (Le(y)) antigens. Also catalyzes the transfer of L-fucose to subterminal GlcNAc of sialyl- and disialyl-lactotetraosylceramide to produce sialyl Lewis a (sLe(a)) and disialyl Lewis a via an alpha(1,4) linkage and therefore may regulate cell surface sialyl Lewis a expression and consequently regulates adhesive properties to E-selectin, cell proliferation and migration. Catalyzes the transfer of an L-fucose to 3'-sialyl-N-acetyllactosamine by an alpha(1,3) linkage, which allows the formation of sialyl-Lewis x structure and therefore may regulate the sialyl-Lewis x surface antigen expression and consequently adhesive properties to E-selectin. Prefers type 1 chain over type 2 acceptors. Type 1 tetrasaccharide is a better acceptor than type 1 disaccharide suggesting that a beta anomeric configuration of GlcNAc in the substrate is preferred. Lewis-positive (Le(+)) individuals have an active enzyme while Lewis-negative (Le(-)) individuals have an inactive enzyme.

Gene References into Functions

High FUT3 expression is associated with breast cancer. PMID:26908442
the high expressions of BCL6 and Lewis y antigen are associated with development, high tumor burden, and worse prognosis of ovarian cancer and targeting BCL6 could be a novel therapeutic strategy for ovarian cancer treatment. PMID:28671040
the miRNA expression vector which targets the FUT3 gene can effectively inhibit the proliferation, migration and invasion abilities of KATO-III cells. PMID:27453266
Polymorphisms in FUT3 and its intestinal expression might be associated with ulcerative colitis pathogenesis. PMID:26766790
Data suggest that fucosyltransferase 3 (FUT3) gene rs28362459 and rs3745635 single nucleotide polymorphisms (SNPs) may engender the increased risk of ileocolonic and ileal Crohn's disease (CD). PMID:26663064
that the absence of alpha1,3/4-fucosyltransferase enzyme is related to breast's invasive breast carcinoma PMID:26321244
Mutations of FUT3 (rs28362459) and (rs3745635) might influence the lesion locations in CD patients. PMID:24720527
Obtained FST values reveal distinct frequencies of the FUT3 SNPs between the present sample and its representative ancestral populations. PMID:23922852
down-regulation of FUT3 and FUT5 reduces the expression of sialyl-Lewis antigens and the adhesion capacities of gastric cancer cells and allows to identify the specific alpha1-3,4 fucosyltransferases implicated in the Lewis antigens synthesis PMID:21978830
Elevated expression of Topo-I and Topo-II beta is found in Lewis(y) antigen-overexpressing ovarian cancer cells. PMID:21542140
LeY is carried by integrin alphavbeta3 and plays a critical role in attachment to cultured cells. LeY activates integrin alphavbeta3/focal adhesion kinase (FAK) signaling. PMID:20605574
At physiological levels of wall shear stress, the number of flowing cancer cells recruited to the microtube surface was dramatically reduced by FUT3 knockdown PMID:20833389
Lewis-negative genotype was associated with invasive ductal breast carcinoma but not with anatomoclinical parameters PMID:20514537
GALNT14 and FUT3/6 H-scores were significantly higher in non-small cell lung cancer cell lines sensitive to dulanermin and drozitumab versus resistant cell lines PMID:20179215
Increased expression of Lewis y antigen plays an important role in promoting cell proliferation through activating PI3K/Akt signaling pathway in ovarian carcinoma-derived RMG-I cells. PMID:20003467
PCR using sequence-specific primers DNA enables efficient genotyping of clinical samples PMID:11668626
Sialy Lewis-X antigens play an important role liver metastases of colon carcinoma. PMID:11819805
Distribution of Lewis (FUT3)genotype and allele frequencies by ethnicity in a United States population. PMID:12424536
Cys, Gln, and Tyr residues are important for FT3wt sorting into the transport vesicles possibly due to interactions with other membrane proteins PMID:12493760
study of the Lewis gene polymorphisms in a Caucasian Portuguese population with a high rate of H. pylori infection, and evaluation of the implications of mutant enzymes in Le(b) expression in the gastric mucosa PMID:12730721
results indicated that Mn2+ bound the enzyme and increased its affinity for the acceptor PMID:14977360
The heterologous enzyme hFUT3 was strongly expressed and fully functional in transgenic tobacco, which showed a delay in growth linked to a reduction of internode length. PMID:15331092
cloning and characterization of the promoter; strong correlation between the promoter activity and high expression of sialyl Le(a) observed in colon carcinoma cell lines seem to confirm the important regulatory role of FUT3 in synthesis of sialyl Le(a) PMID:16199102
LeX motif present in human milk can bind to dendritic cell-specific ICAM3-grabbing non-integrin (DC-SIGN), thereby preventing the capture and subsequent transfer of HIV-1 to CD4+ T lymphocytes. PMID:16239964
T3 overexpression in gastric cells depends upon promoter hypomethylation and FUT3 is responsible for overexpression of Le(a) in gastric cells, in vitro PMID:16427187
functional mutations of the FUT3 gene may be associated with an increased atherothrombotic disease prevalence, especially among abstainers PMID:17383304
increased CD15 expression is not due to de novo biosynthesis of CD15, but results predominantly from induction of alpha(2-3)-sialidase activity PMID:18953356
Expression of sLex antigen in breast cancer is not associated with breast cancer survival. PMID:18977761
These findings indicate that Lewis y antigen have the ability to enhance the proliferation and elevate the survival rates of RMG-I cells, which can promote the genesis and development of ovarian carcinoma PMID:19137814
Sequence analysis of FUT3 identified many SNPs, but most of them are in complete linkage disequilibrium with previously reported SNPs responsible for the Lewis-negative phenotype. PMID:19175549
results suggested that PKR is the primary target for HSV-1 early RNA during induction of FUT3, FUT5, and FUT6 PMID:19349624
in breast cancer there was a limited humoral immune response through Lewis y/IgM/CIC levels detection which correlated with MUC1/IgM/CIC PMID:19715603