EPHA5 Antibody

Code: CSB-PA007725LA01HU

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US$83

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Species Reactivity: Human

Raised in: Rabbit

Application: ELISA, WB, IHC, IF

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Target Background

Product Details

Uniprot NO.

P54756

Target Names

EPHA5

Alternative Names

Brain-specific kinase antibody; bsk antibody; CEK 7 antibody; Eck like sequence 1 antibody; EHK 1 antibody; EHK-1 antibody; EK7 antibody; Els 1 antibody; EPH homology kinase 1 antibody; EPH-like kinase 7 antibody; EPHA5 antibody; EPHA5_HUMAN antibody; Ephrin type A receptor 5 antibody; Ephrin type-A receptor 5 antibody; HEK 7 antibody; hEK7 antibody; Receptor protein tyrosine kinase HEK 7 antibody; Rek 7 antibody; TYRO 4 antibody; Tyrosine protein kinase receptor EHK 1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Ephrin type-A receptor 5 protein (246-514AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate
All lanes: EPHA5 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 115, 113, 108 kDa
Observed band size: 115 kDa
Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA007725LA01HU at dilution of 1:100
Immunofluorescent analysis of A549 cells using CSB-PA007725LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Description

CUSABIO's product CSB-PA007725LA01HU is a polyclonal antibody against EPHA5. It is synthesized in a rabbit immunized with the recombinant human EPHA5 protein (246-514aa). After protein G affinity chromatography purification, this EPHA5 antibody reaches up to 95% in purity. This EPHA5 antibody can react with human EPHA5 protein. CUSABIO tested its specificity for the EPHA5 protein in ELISA, WB, IHC, and IF applications.

EPHA5 mediates cell signaling pathways that regulate cellular adhesion, migration, and survival. Specifically, EPHA5 is involved in regulating cell repulsion and attraction and plays a critical role in controlling axon guidance during neural development. Aberrant expression or activity of EPHA5 has been implicated in several diseases, including cancer, neurodegenerative disorders, and psychiatric disorders.

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Target Background

Function(From Uniprot)

Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino-hippocampal and hippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis. In addition to its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion.

Gene References into Functions

Gene-based analysis identified EPHA6 as the gene most significantly associated with paclitaxel-induced neuropathy...This first study sequencing EPHA genes revealed that low-frequency variants in EPHA6, EPHA5, and EPHA8 contribute to the susceptibility to paclitaxel-induced neuropathy PMID:27582484
Our data indicate that EphA5 receptor may be a tumor suppressor in colorectal carcinoma and it may be a new therapeutic target for colorectal carcinoma. PMID:27651378
EphA5 protein was negatively (0) or weakly (1+) expressed in 48 of 78 (61.5%), moderately (2+) expressed in 15 of 78 (19.2%) and strongly (3+) expressed in 15 of 78 (19.2%) tumour samples of clear cell renal cell carcinoma (ccRCC). Decreased expression of EphA5 was detected more often in females than in males PMID:28421649
the interactions of EphA5/ephrinA5 and/or EphA7/ephrinA5 between HSPCs and BMSCs, independently and cooperatively, play a role in HSPC colony formation through the upregulation of GM-CSFR. Furthermore, the adhesion/migration of HSPCs appears to be mediated in part through the activation of Rac1. PMID:27988259
Our results show that EphA5 may be a potential biomarker for distinguishing high-and low-grade ovarian serous carcinoma and a potential prognostic marker. PMID:27887627
Our study provides evidence that EphA5 is a potential target for epigenetic silencing in primary prostate cancer and is a potentially valuable prognosis predictor and thereapeutic marker for prostate cancer. PMID:25609195
demonstrate that a new monoclonal antibody against human EphA5 sensitized lung cancer cells and human lung cancer xenografts to radiotherapy and significantly prolonged survival, thus suggesting the likelihood of translational applications PMID:25623065
unbound EphA5 LBD appears to comprise an ensemble of open conformations that have only small variations over the loops and appear ready to bind ephrin-A ligands PMID:24086308
These results indicate that EphA5 may be a negative regulator of bone formation. PMID:24029132
Insertional translocation leading to a 4q13 duplication including the EPHA5 gene is associated with attention-deficit. hyperactivity disorder PMID:23824631
these data suggest that miR-34a is a negative modulator of chondrogenesis, particularly in migration of chondroblasts, by targeting EphA5 and resulting inhibition of cellular condensation during chondrogenesis of chick limb mesenchymal cells. PMID:22079638
Eph-A5 and Eph-A7 staining intensity was identified as independent prognostic factors for pancreatic ductal adenocarcinoma PMID:19949912
EphA5 might be a potential target for epigenetic silencing in primary breast cancer and a valuable molecular marker for breast cancer carcinogenesis and progression. PMID:19733895

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Subcellular Location

Cell membrane; Single-pass type I membrane protein. Cell projection, axon. Cell projection, dendrite.

Protein Families

Protein kinase superfamily, Tyr protein kinase family, Ephrin receptor subfamily

Tissue Specificity

Almost exclusively expressed in the nervous system in cortical neurons, cerebellar Purkinje cells and pyramidal neurons within the cortex and hippocampus. Display an increasing gradient of expression from the forebrain to hindbrain and spinal cord.

Database Links

HGNC: 3389

UNIGENE: Hs.654492

KEGG: hsa:2044

STRING: 9606.ENSP00000273854

OMIM: 600004

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