CHRM5 Antibody

Code: CSB-PA005385LA01HU

Rate （N/A）
Review （0）

Size:

20μg50μg100μg

US$83

Quantity:

Species Reactivity: Human, Mouse

Raised in: Rabbit

Application: ELISA, WB, IHC, IF

Send an Inquiry

Start an on-line Chat

Online ordering is currently available for U.S. customers only. For orders outside the U.S., please kindly submit an inquiry or start a chat with us.

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot NO.

P08912

Target Names

CHRM5

Alternative Names

CHRM5; Muscarinic acetylcholine receptor M5

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Muscarinic acetylcholine receptor M5 protein (220-427AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: Mouse liver tissue, Mouse kidney tissue, Mouse brain tissue
All lanes: CHRM5 antibody at 3.5μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA005385LA01HU at dilution of 1:100
Immunofluorescent analysis of A549 cells using CSB-PA005385LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Customer Reviews and Q&A

■ Customer Reviews

Submit a Review here

Target Background

Function(From Uniprot)

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

Gene References into Functions

Immunological stimulation leads to M5 mAChR gene expression in lymphocytes. PMID:12675126
direct evidence suggesting that the M5 muscarinic receptor gene combined with the alpha7-nicotinic receptor gene may be linked to schizophrenia PMID:15292665
mAChR subtypes (m1 to m5)presents in human scleral fibroblasts at both mRNA and protein levels. PMID:16877267
the first study to show an association between CHRM5 and substance use in humans PMID:17608938