CDK12 Antibody

Code: CSB-PA998940

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Size:

50μl100μl

US$166

Quantity:

Species Reactivity: Human,Mouse,Rat

Raised in: Rabbit

Application: ELISA,IHC

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Product Details
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Target Background

Product Details

Uniprot NO.

Q9NYV4

Target Names

CDK12

Alternative Names

arginine/serine-rich antibody; Cdc2 related kinase arginine/serine rich antibody; CDC2 related protein kinase 7 antibody; Cdc2-related kinase antibody; CDC2-related protein kinase 7 antibody; Cdk12 antibody; CDK12_HUMAN antibody; Cell division cycle 2 related protein kinase 7 antibody; Cell division cycle 2-related protein kinase 7 antibody; Cell division protein kinase 12 antibody; CRK7 antibody; CRKR antibody; CrkRS antibody; Cyclin-dependent kinase 12 antibody; EC 2.7.11.22 antibody; hCDK12 antibody; kiaa0904 antibody; Pksc antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Synthetic peptide of Human CDK12

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA,IHC

Protocols

ELISA Protocol Immunohistochemistry (IHC) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using CSB-PA998940(CDK12 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using CSB-PA998940(CDK12 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200)

Customer Reviews and Q&A

■ Customer Reviews

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Target Background

Function(From Uniprot)

Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors.

Gene References into Functions

Cyclin K expression positively correlates with cell proliferation; knockdown of cyclin K or its cognate kinase CDK12 prevents the assembly of prereplicative complex in the G1 cell cycle phase. PMID:29760377
CDK12 regulates prereplicative complex assembly to promote mammalian cell proliferation. PMID:29760377
CDK12 gene amplification can contribute to the pathogenesis of breast cancer. PMID:28334900
BRCA1 downregulation combined with CHK1 inhibition induced excessive amounts of DNA damage, resulting in an inability to complete the S-phase. Therefore, we suggest CHK1 inhibition as a strategy for targeting BRCA1- or CDK12-deficient tumors. PMID:29025359
In Ovarian Cancer patients CDK12 loss is consistently associated with a particular genomic instability pattern characterized by hundreds of tandem duplications PMID:26787835
Structures of CDK12/CycK complexes solved in the presence of AMP-PNP. PMID:26597175
Data show that most mutations prevent formation of the cyclin-dependent kinase 12 (Cdk12)/cyclin K (CycK) complex, rendering the kinase inactive. PMID:25712099
CDK12 affects RNA processing events in two distinct ways: Indirectly through generating factor-binding phospho-epitopes on the CTD of elongating RNAPII and directly through binding to specific factors PMID:25429106
CDK12 and CDK13 losses in HCT116 cells preferentially affect expression of DNA damage response. PMID:25561469
CDK12 plays an important role in cotranscriptional processing of c-FOS transcripts PMID:25384976
CDK12 has properties that should confirm interest in its use as a biomarker. PMID:24240700
many CDK12 mutations are an unrecognized cause of HR defects in ovarian cancers PMID:24554720
CDK12 interacts with cyclin K and is required to maintain embryonnic stem cell self-renewal. PMID:22547058
Cyclin K1 is the primary cyclin partner for CDK12/CrkRS and it is required for activation of CDK12/CrkRS to phosphorylate the C-terminal domain of RNA Pol II. PMID:22988298
through regulation of expression of DNA damage response genes, CycK/Cdk12 protects cells from genomic instability PMID:22012619
Data show that siRNA knockdown of hCDK12 in HeLa cells results in alterations in the CTD phosphorylation state. PMID:20952539
CRK7 modifies MAP kinases regulation and resistance to estrogen signaling inhibitors in breast cancers. PMID:19651820