CARD6 Antibody

Code: CSB-PA863138LA01HU

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Size:

20μl50μl100μl

US$83

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Species Reactivity: Human

Raised in: Rabbit

Application: ELISA, IHC, IF

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Product Details
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Target Background

Product Details

Uniprot NO.

Q9BX69

Target Names

CARD6

Alternative Names

CARD 6 antibody; CARD containing inhibitor of Nod1 and Cardiak-induced NF kB activation antibody; CARD6 antibody; CARD6_HUMAN antibody; Caspase recruitment domain family; member 6 antibody; Caspase recruitment domain protein 6 antibody; Caspase recruitment domain-containing protein 6 antibody; CINCIN1 antibody; D730008L15 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Caspase recruitment domain-containing protein 6 protein (666-930AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-4 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

IHC image of CSB-PA863138LA01HU diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of U251 cells with CSB-PA863138LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Target Background

Function(From Uniprot)

May be involved in apoptosis.

Gene References into Functions

The increased expression of CARD6 in esophageal squamous cell carcinoma,gastric and colorectal adenocarcinoma suggested that neoexpression of CARD6 might be related to activation of NF-kappaB pathway. PMID:20025480
identification as a modulator of NF-kappa B activation by Nod1- and Cardiak-mediated pathways PMID:12775719
CARD6 is a regulator of NF-kappaB activation that modulates the functions of RICK protein PMID:16418290
review of the regulation of interactions of CARD6 with RICK and microtubules [review] PMID:16582588