BRD9 Antibody

Code: CSB-PA888000ESR2HU

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Size:

20μl50μl100μl

US$83

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Species Reactivity: Human, Mouse

Raised in: Rabbit

Application: ELISA, WB, IHC, IF

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Target Background

Product Details

Uniprot NO.

Q9H8M2

Target Names

BRD9

Alternative Names

BRD9 antibody; UNQ3040/PRO9856Bromodomain-containing protein 9 antibody; Rhabdomyosarcoma antigen MU-RMS-40.8 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Bromodomain-containing protein 9 protein (338-597AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:200-1:1000
IHC	1:20-1:200
IF	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western blot
All lanes: Bromodomain-containing protein 9 antibody at 2μg/ml + Mouse liver tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 68, 23, 54, 27, 56, 61 kDa
Observed band size: 68 kDa
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA888000ESR2HU at dilution of 1:100
Immunofluorescent analysis of HepG2 cells using CSB-PA888000ESR2HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Description

A partial recombinant protein of the human BRD9 protein, corresponding to the 338-597 amino acids, was used to immunize the rabbit to synthesize the BRD9 antibody. The resulting BRD9 antibody is an unconjugated IgG isoform that has undergone antigen affinity purification.

To ensure its reliability, this BRD9 polyclonal antibody has been rigorously quality controlled in multiple applications, including ELISA, WB, IHC, and IF. It has been shown to be reactive with human and mouse BRD9 proteins. The BRD9 protein is a subunit of the BAF chromatin remodeling complex, which plays a crucial role in regulating gene expression and chromatin structure. Additionally, BRD9 has been implicated in other cellular processes, such as cell division, DNA damage repair, and cell differentiation.

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Target Background

Function(From Uniprot)

Plays a role in chromatin remodeling and regulation of transcription. Acts as a chromatin reader that recognizes and binds acylated histones: binds histones that are acetylated and/or butyrylated. Component of SWI/SNF chromatin remodeling subcomplex GBAF that carries out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Orchestrates also the RAD51-RAD54 complex formation and thereby plays a role in homologous recombination (HR).

Gene References into Functions

The bromodomains of BRD9, CECR2, and the second bromodomain of TAF1 recognize the longer butyryl mark on histones. In addition, the TAF1 second bromodomain is capable of binding crotonyl marks. PMID:26365797

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Database Links

HGNC: 25818

UNIGENE: Hs.449278

KEGG: hsa:65980

STRING: 9606.ENSP00000419765

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