Abbreviation
Recombinant Cynomolgus monkey IL6 protein (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is 0.0541-0.2317 ng/mL.
Alternative Names
Interleukin-6; IL-6
Species
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Expression Region
28-212aa
Target Protein Sequence
APVLPGEDSKDVAAPHSQPLTSSERIDKHIRYILDGISALRKETCNRSNMCESSKEALAENNLNLPKMAEKDGCFQSGFNEDTCLVKIITGLLEFEVYLEYLQNRFESSEEQARAVQMSTKVLIQFLQKKAKNLDAITTPEPTTNASLLTKLQAQNQWLQDMTTHLILRSFKEFLQSSLRALRQM
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-81°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
-
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
-
Activity
The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is 0.0541-0.2317 ng/mL.
Description
Interleukin-6 drives pleiotropic signaling in immune and cancer biology, making precise control of its bioactivity essential for dissecting pathway-specific outcomes in cynomolgus macaque models. This recombinant protein demonstrates dose-dependent proliferative activity on human TF-1 cells with an ED50 of 0.0541–0.2317 ng/mL, providing quantitative evidence of functional potency suitable for cell-based proliferation assays, JAK/STAT pathway activation studies, and differentiation experiments where IL-6–dependent responses must be reliably induced. The endotoxin level of less than 1.0 EU/μg minimizes LPS-driven artifacts that can confound interpretation in primary immune cell cultures and in vivo inflammation models. Spanning the full mature protein sequence (aa 28–212) and exceeding 95% purity by SDS-PAGE, this material supports antibody development workflows, including immunogen preparation and ELISA standard curve generation, where sequence authenticity and low contaminant burden are critical for reproducible results.
