Abbreviation
Recombinant Human MIA protein (Active)
Purity
>98% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human A375 cell line is less than 5 μg/ml, corresponding to a specific activity of >200 IU/mg.
Alternative Names
Cartilage derived retinoic acid sensitive protein; CD RAP; CDRAP; Melanoma derived growth regulatory protein precursor; Melanoma inhibitory activity protein; Melanoma-derived growth regulatory protein; Mia; MIA_HUMAN
Species
Homo sapiens (Human)
Expression Region
25-131aa
Target Protein Sequence
GPMPKLADRK LCADQECSHP ISMAVALQDY MAPDCRFLTI HRGQVVYVFS KLKGRGRLFW GGSVQGDYYG DLAARLGYFP SSIVREDQTL KPGKVDVKTD KWDFYCQ
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
0.2 µm filtered concentrated solution in PBS, pH 7.4, with 5 % Trehalose,lyophilized
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Description
MIA functions as a secreted factor in melanoma biology, modulating cell proliferation and tumor microenvironment interactions—making validated recombinant forms essential for mechanistic studies. This tag-free protein spans residues 25–131, representing the full-length mature sequence, and demonstrates an ED50 of less than 5 μg/ml in A375 cell proliferation assays, corresponding to a specific activity exceeding 200 IU/mg. Because MIA lacks glycosylation sites critical to its function, E. coli expression yields a biologically active protein suitable for receptor-ligand binding studies via SPR or ELISA, tumor biology models, and use as a quantitative standard in antibody characterization assays. Purity exceeding 98% by SDS-PAGE combined with endotoxin levels below 1.0 EU/μg satisfies the criteria typical for cell-based proliferation and survival assays without confounding inflammatory artifacts.
