Abbreviation
Recombinant Human CLDN18.2 protein-Detergent (Active)
Purity
Greater than 80% as determined by SDS-PAGE.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CLDN18.2 at 5 μg/mL on an Nickel Coated plate can bind Anti-CLDN18.2 recombinant antibody (CSB-RA005505MA1HU), the EC50 is 0.4640-0.6372 ng/mL.
Alternative Names
(CLDN18.2)
Species
Homo sapiens (Human)
Expression Region
1-261aa
Target Protein Sequence
MAVTACQGLGFVVSLIGIAGIIAATCMDQWSTQDLYNNPVTAVFNYQGLWRSCVRESSGFTECRGYFTLLGLPAMLQAVRALMIVGIVLGAIGLLVSIFALKCIRIGSMEDSAKANMTLTSGIMFIVSGLCAIAGVSVFANMLVTNFWMSTANMYTGMGGMVQTVQTRYTFGAALFVGWVAGGLTLIGGVMMCIACRGLAPEETNYKAVSYHASGHSVAYKPGGFKASTGFGSNTKNKKIYDGGARTEDEVQSYPSKHDYV
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Isoform A2
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
50 mM HEPES，0.15 M NaCl， 0.05% DDM，0.01% CHS，pH7.5
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CLDN18.2 at 5 μg/ml on an Nickel Coated plate can bind Anti-CLDN18.2 recombinant antibody (CSB-RA005498A1HU), the EC50 is 0.4640-0.6372 ng/mL.
Description
Claudin-18.2 represents a gastric-specific tight junction protein that has emerged as a promising target in oncology, particularly for gastric and pancreatic adenocarcinomas where aberrant expression correlates with tumor progression. This full-length isoform A2 construct (aa 1–261) expressed in mammalian cells demonstrates robust antibody binding activity in functional ELISA, achieving an EC50 of 0.46–0.64 ng/mL with an anti-CLDN18.2 recombinant antibody—a quantitative measure that supports its use in antibody development and validation workflows where precise epitope recognition is critical. The mammalian expression system preserves native post-translational modifications and membrane protein topology, providing a suitable basis for cell adhesion assays and tumor metastasis research models that depend on authentic claudin-mediated cell–cell interactions. Supplied with a C-terminal 10×His tag for streamlined purification and immobilization, this protein meets the >80% purity threshold commonly required for functional binding studies and therapeutic antibody screening campaigns.
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