Abbreviation
Recombinant Human CD52 protein-Nanoparticle (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA.Immobilized Human CD52 at 2 μg/mL can bind Anti-CD52 recombinant antibody (CSB-RA004943MA1HU).The EC50 is 199.3-226.1 ng/mL.
Alternative Names
CAMPATH-1 antigen; CDw52; Cambridge pathology 1 antigen; Epididymal secretory protein E5; Human epididymis-specific protein 5 (He5); CD52; CDW52, HE5
Species
Homo sapiens (Human)
Expression Region
25-36aa
Target Protein Sequence
GQNDTSQTSSPS
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-81°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA.Immobilized Human CD52 at 2 μg/ml can bind Anti-CD52 recombinant antibody (CSB-RA004943MA1HU).The EC50 is 199.3-226.1 ng/mL.
Description
CD52, a GPI-anchored glycoprotein abundantly expressed on lymphocytes and targeted by alemtuzumab in leukemia therapy, presents unique challenges for recombinant production due to its minimal 12-amino acid extracellular domain. This mammalian-expressed protein spans the complete mature sequence (aa 25–36) and demonstrates quantifiable binding to anti-CD52 antibodies with an EC50 of 199.3–226.1 ng/mL in functional ELISA, confirming that the native epitope architecture is preserved despite the protein's exceptionally small size. The validated antibody recognition supports its use in therapeutic antibody development programs, epitope mapping studies, and competitive binding assays designed to characterize CD52-targeting biologics. With purity exceeding 95% by SDS-PAGE and endotoxin levels below 1.0 EU/μg, this protein meets the quality thresholds commonly required for cell-based assays investigating lymphocyte depletion mechanisms and for screening next-generation antibody candidates against this clinically validated cancer target.
