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Recombinant Human Asialoglycoprotein receptor 1 (ASGR1)-Detergent (Active)

Recombinant Human Asialoglycoprotein receptor 1 (ASGR1)-Detergent (Active)

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Code: CSB-MP002207HU(A4)-D

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20ug100ug1mg

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Express system: Mammalian cell

Species: Homo sapiens (Human)

Tag Info: C-terminal 10xHis-tagged

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Target Background

Product Details

Abbreviation

Recombinant Human ASGR1 protein-Detergent (Active)

Purity

Greater than 85% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

①Measured by its binding ability in a functional ELISA. Immobilized Human ASGR1 at 2 μg/mL on an Nickel Coated plate can bind Anti-ASGR1 recombinant antibody (CSB-RA002207MA3HU). The EC50 is 2.715-3.605 ng/mL. ②ASGR1 Recombinant Monoclonal Antibody (CSB-RA002207MA3HU) captured on Protein A Chip can bind Recombinant Human ASGR1 with an affinity constant of 0.716 nM as detected by MetaSPR Assay (WeSPRTM 200).

Target Names

ASGR1

Uniprot NO.

P07306

Alternative Names

Asialoglycoprotein receptor 1; ASGP-R 1; ASGPR 1; C-type lectin domain family 4 member H1; Hepatic lectin H1; HL-1

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

1-291aa

Target Protein Sequence

MTKEYQDLQHLDNEESDHHQLRKGPPPPQPLLQRLCSGPRLLLLSLGLSLLLLVVVCVIGSQNSQLQEELRGLRETFSNFTASTEAQVKGLSTQGGNVGRKMKSLESQLEKQQKDLSEDHSSLLLHVKQFVSDLRSLSCQMAALQGNGSERTCCPVNWVEHERSCYWFSRSGKAWADADNYCRLEDAHLVVVTSWEEQKFVQHHIGPVNTWMGLHDQNGPWKWVDGTDYETGFKNWRPEQPDDWYGHGLGGGEDCAHFTDDGRWNDDVCQRPYRWVCETELDKASQEPPLL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

34.6 kDa

Protein Length

Full Length

Tag Info

C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Buffer

Lyophilized from a 0.2 μm sterile filtered 50 mM HEPES, 0.15 M NaCl, 0.05% DDM, 0.01% CHS, 6% Trehalose, pH 7.5

Storage

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Lead Time

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Shelf Life

The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.

Troubleshooting and FAQs

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Datasheet & COA

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Images

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human ASGR1 at 2 μg/ml on an Nickel Coated plate can bind Anti-ASGR1 recombinant antibody (CSB-RA002207MA3HU). The EC₅₀ is 2.715-3.605 ng/mL.
Activity
ASGR1 Recombinant Monoclonal Antibody (CSB-RA002207MA3HU) captured on Protein A Chip can bind Recombinant Human ASGR1 with an affinity constant of 0.716 nM as detected by MetaSPR Assay (WeSPRTM 200).

Description

ASGR1 mediates the endocytosis of desialylated glycoproteins in hepatocytes, a pathway exploited for liver-targeted drug delivery and implicated in metabolic regulation and hepatocellular carcinoma progression. This full-length construct (aa 1–291) expressed in mammalian cells preserves native glycosylation and disulfide architecture, as evidenced by sub-nanomolar affinity (KD = 0.716 nM) to a validated monoclonal antibody measured by surface plasmon resonance and an EC50 of 2.715–3.605 ng/mL in functional ELISA. The demonstrated binding kinetics support use in therapeutic antibody epitope mapping, competitive inhibition screening for liver-targeting biologics, and ligand-receptor interaction studies by SPR or biolayer interferometry. With purity exceeding 85% by SDS-PAGE and endotoxin below 1.0 EU/μg, this protein aligns with standards expected in antibody validation workflows and small-molecule inhibitor discovery campaigns targeting hepatic lectin pathways.

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Target Background

Function(From Uniprot)

Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.

Gene References into Functions

Authors report the generation of a human embryonic stem cell line WAe001-A-6 harbouring homozygous ASGR1 mutations using CRISPR/Cas9. The mutation involves a 37bp deletion, resulting in a frame shift. PMID:28952928
PEGylated lipoplexes were well tolerated by both HEK293 (ASGP-R-negative) and HepG2 (ASGP-R-positive) cell lines and delivered DNA to the hepatoma cell line HepG2 by ASGP-R mediation at levels three-fold greater than nonPEGylated lipoplexes. PMID:28063265
findings show that the hepatitis E virus (HEV) ORF2 protein interacts directly with the ectodomain of both ASGR1 and ASGR2; ASGPR is involved in and facilitates HEV infection by binding to ORF2 PMID:27155063
ASGR1 can inhibit the activity of V-ATPase by interacting with LASS2, thereby suppressing the metastatic potential of hepatoma cells. PMID:27241665
This review will focus on the mechanisms of platelet senescence with specific emphasis on the role of post-translational modifications in platelet life-span and thrombopoietin production downstream of the hepatic Ashwell-Morrell receptor, originally termed asialoglycoprotein receptor (ASGPR). PMID:27207430
ASGR1 haploinsufficiency was associated with reduced levels of non-HDL cholesterol and a reduced risk of coronary artery disease. PMID:27192541
Flow cytometric assessment of ASGPR expression may be a useful predictor of liver dysfunction following major hepatectomy for HCC in Chinese patients. PMID:25404440
Anti-ASGPR antibody could be used for specific and efficient HCC CTC enrichment, and anti-P-CK combined with anti-CPS1 antibodies is superior to identification with one antibody alone in the sensitivity for HCC CTC detection. PMID:24763545
Endocytic AMR controls TPO expression through Janus kinase 2 (JAK2) and the acute phase response signal transducer and activator of transcription 3 (STAT3) in vivo and in vitro PMID:25485912
Anti-ASGPR levels correlate with biochemical parameters and with the severity and manifestation autoimmune processes in patients with autoimmune hepatitis. PMID:24933948
The examined ASGPR1 expression levels by immunohistochemistry in HCC with different grades. Guidance for a targeting delivery strategy for anti-cancer drugs to HCC is suggested in this report. PMID:23979840
the distribution of ASGR in human testis, was investigated. PMID:23604802
the cooperative binding mode of Ca(2+) makes it possible for ASGP-R to be more sensitive to Ca(2+) concentrations in early endosomes, and plays an important role in the efficient release of ligand from ASGP-R PMID:22613667
Constant sH2a levels suggest constitutive secretion from hepatocytes in healthy individuals; therefore, a decrease with cirrhosis suggests a diagnostic potential. PMID:22219600
found that the asialoglycoprotein receptor (ASGPR) is involved in hepatocyte recognition of cells predestined for killing, including activated autologous T lymphocytes PMID:21656538
Asialoglycoprotein receptor (ASGPR) interacted specifically and directly with the preS1 domain of HBV in vivo and in vitro. PMID:21207081
Two naturally occurring ASGPR1 splice variants are produced in human hepatocytes. PMID:20886072
our findings suggest that both fibronectin and ASGPR mediate HBsAg binding to the cell surface, which provides further evidence for the potential roles of these two proteins in mediating HBV binding to liver cells. PMID:20364278
The minor subunit splice variants, H2b and H2c, of the human asialoglycoprotein receptor are present with the major subunit H1 in different hetero-oligomeric receptor complexes PMID:11943787
primary renal proximal tubular epithelial cells have a functional ASGPR, consisting of the H1 and H2 subunits, that is capable of specific ligand binding and uptake PMID:12119473
Phosphorylation-dependent interaction with molecular chaperones PMID:12167617
the effects of palmitoylation on ASGP-R activity and function PMID:12359251
the spacing of a Cys residue relative to the TMD in the primary protein sequence of H1 is the major determinant for successful palmitoylation PMID:12370180
Exposure of beta-galactose results in the rapid clearance of platelets from the circulation by asialoglycoprotein receptor-expressing liver macrophages and hepatocytes. PMID:19520807