Abbreviation
Recombinant Human ALPP protein (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Greater than 95% as determined by SEC-HPLC.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human ALPP at 2 μg/mL can bind Anti-ALPP/ALPG recombinant antibody (CSB-RA001633MA1HU). The EC50 is 4.478-5.236 ng/mL.
Alternative Names
Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1
Species
Homo sapiens (Human)
Expression Region
23-506aa
Target Protein Sequence
IIPVEEENPDFWNREAAEALGAAKKLQPAQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKHVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Human ALPP at 2 μg/ml can bind Anti-ALPP/ALPG recombinant antibody (CSB-RA001633MA1HU). The EC50 is 4.478-5.236 ng/mL.
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The purity of ALPP was greater than 95% as determined by SEC-HPLC
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ALPG Recombinant Monoclonal Antibody (CSB-RA001633MA1HU) captured on Protein A Chip can bind Recombinant Human ALPP protein with an affinity constant of 0.796 nM as detected by MetaSPR Assay (WeSPRTM 200).
Description
Placental alkaline phosphatase serves as a tumor marker in several malignancies and exhibits distinct biochemical properties compared to tissue-nonspecific isoforms, making it a valuable target for cancer research and diagnostic development. This full-length mature protein (aa 23–506) with C-terminal 10xHis tag demonstrates functional integrity in binding assays, achieving an EC50 of 4.478–5.236 ng/mL when immobilized at 2 μg/mL and tested against anti-ALPP recombinant antibody, confirming proper folding and epitope accessibility. The greater than 95% purity determined by SDS-PAGE, combined with endotoxin levels below 1.0 EU/μg, meets the quality thresholds commonly required for kinetic parameter analysis (Km, Vmax, kcat) and inhibitor screening assays where contaminants would confound IC50 determinations. Researchers can apply this preparation as a positive control in enzyme-linked assays, for substrate specificity profiling, or in drug candidate evaluation studies targeting alkaline phosphatase activity in oncology contexts.
