Abbreviation
Recombinant Human ALPG protein (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
①Measured by its binding ability in a functional ELISA. Immobilized Human ALPG at 2 μg/mL can bind Anti-ALPG recombinant antibody (CSB-RA001633MA1HU), the EC50 is 14.09-23.17 ng/mL.
Alternative Names
(ALP-1)(Alkaline phosphatase Nagao isozyme)(Alkaline phosphatase, placental-like)(Germ cell alkaline phosphatase )(Placental alkaline phosphatase-like)
Species
Homo sapiens (Human)
Expression Region
20-503aa
Target Protein Sequence
IIPVEEENPDFWNRQAAEALGAAKKLQPAQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPETFLAMDRFPYVALSKTYSVDKHVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTRVQHASPAGAYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYMFPMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKHQGARYVWNRTELLQASLDPSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALLLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDGETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPRAGTTD
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Human ALPG at 2μg/mL can bind Anti-ALPG recombinant antibody (CSB-RA001633MA1HU)， the EC50 is 14.09-23.17 ng/mL.
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ALPG Recombinant Monoclonal Antibody (CSB-RA001633MA1HU) captured on Protein A Chip can bind Recombinant Human ALPG protein with an affinity constant of 0.444 nM as detected by MetaSPR Assay (WeSPRTM 200).
Description
ALPG, a germ cell-specific alkaline phosphatase isoenzyme overexpressed in certain malignancies, serves as both a cancer biomarker and a functional enzyme target in oncology research. This full-length mature protein (aa 20–503) with C-terminal 10xHis tag demonstrates quantifiable binding activity in functional ELISA, achieving an EC50 of 14.09–23.17 ng/mL with anti-ALPG recombinant antibody, which supports its use as a positive control in enzyme-linked immunoassays and validates its structural integrity for antibody-antigen interaction studies. The protein meets the purity and endotoxin criteria typical for inhibitor screening and IC50 determination experiments, with >95% purity by SDS-PAGE and <1.0 EU/μg endotoxin, providing a suitable basis for drug candidate evaluation where low contaminant levels are essential to avoid interference in dose-response measurements. Researchers investigating ALPG's role in germ cell tumors or placental-like alkaline phosphatase expression in cancer can apply this reagent to substrate specificity profiling and kinetic parameter analysis once enzymatic activity against phosphate substrates is characterized in their assay conditions.
