CD14 Monoclonal Antibody

Code: CSB-MA004879A0m

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Size:

50μl100μl

US$210

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Species Reactivity: Human, Mouse, Rabbit

Raised in: Mouse

Application: ELISA, WB, IF, FC

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Target Background

Product Details

Uniprot NO.

P08571

Target Names

CD14

Alternative Names

CD 14 antibody; CD_antigen=CD14 antibody; CD14 antibody; CD14 antigen antibody; CD14 molecule antibody; CD14_HUMAN antibody; LPS-R antibody; Mo2 antibody; Monocyte differentiation antigen CD14 antibody; Monocyte differentiation antigen CD14 urinary form antibody; Monocyte differentiation antigen CD14; membrane-bound form antibody; Myeloid cell specific leucine rich glycoprotein antibody; Myeloid cell-specific leucine-rich glycoprotein antibody

Raised in

Mouse

Species Reactivity

Human, Mouse, Rabbit

Immunogen

Recombinant Human Monocyte differentiation antigen CD14 protein (20-345AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

IgG1

Clone No.

14G1F3

Purification Method

>95%, Protein A purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IF	1:50-1:200

Protocols

ELISA Protocol Western Blotting(WB) Protocol Immunofluorescence (IF) Protocol Flow Cytometry (FC) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: Rabbit spleen tissue, Rabbit small intestine tissue
All lanes: CD14 antibody at 1:2500
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 41 kDa
Observed band size: 55 kDa
Western Blot
Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate
All lanes: CD14 antibody at 1:1800
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 41 kDa
Observed band size: 55 kDa
Immunofluorescence staining of A549 cells with CSB-MA004879A0m at 1:90, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescence staining of Hela cells with CSB-MA004879A0m at 1:90, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescence staining of HepG2 cells with CSB-MA004879A0m at 1:90, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescence staining of RAW264.7 cells with CSB-MA004879A0m at 1:90, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescence staining of SY5Y cells with CSB-MA004879A0m at 1:90, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Overlay histogram showing Jurkat cells stained with CSB-MA004879A0m (red line) at 1:180. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
Overlay histogram showing RAW264.7 cells stained with CSB-MA004879A0m (red line) at 1:180. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

Description

This product is the antibody raised in mouse against the human CD14 on the region 20-345aa, which is the mature extracellular portion of the protein in its membrane-bound GPI-anchor form. This monoclonal antibody exists as a non-conjugated isotype IgG1 purified by protein A with a purity greater than 95% and has reactivity against the CD14 from human, mouse, and rabbit. This antibody can be used in ELISA, Western blot (WB), Flow Cytometry (FC), Immunofluorescence (IF), and Immunohistochemistry (IHC), in immunology studies for characterization of disease immune pathways and how the immune system engages in pro-inflammatory processes caused by pathogens. The human monocyte differentiation antigen CD14 participates in the activation of the innate immune response. The CD14 protein is detected in the surface of monocytes and by most tissue macrophages. CD14 regulates immune response against bacteria by recognizing the bacterial LPS and also induces cellular and immune responses against diacylated and triacylated lipopeptides, and electronegative low-density lipoprotein (LDL^-).

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Target Background

Function(From Uniprot)

Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-).

Gene References into Functions

high plasma levels of sCD14 is associated with measles virus infection. PMID:29795672
a considerable connection of DEFB1 and TCF7L2 gene polymorphisms with nephrolithiasis PMID:29959006
CD14 C-(260)T polymorphism is not associated with incidence of acute myocardial infarction in Egyptians who showed elevated serum CD14 levels in PMID:29874995
This study provides better understanding of the mechanisms and disease susceptibility for MIF and CD14 genetic variants and inflammatory miRNAs networks involved in ankylosing spondylitis and polyarthralgia. PMID:29863307
Childhood-onset and adult-onset of asthma showed significant difference in allergen sensitivity as well as genetic background with respect to CD14 polymorphism. PMID:29937881
Renal transplant recipients carrying the CD14-159 TT genotype have significantly higher risk of acute rejection and reduced transplant survival rate than patients with heterozygous or wild-type genotypes. PMID:28411360
The accumulating evidence does not support an association of CD14 SNP rs2569190 with AR risk. [meta-analysis] PMID:29687183
It seems that CD14 gene polymorphism might be associated with the risk of CAD, whereas COL4A1 gene polymorphism was not found to confer any risk of CAD PMID:29299748
higher sCD14 levels in HIV-positive women were associated with a more compromised maternal immunological status and to a lower neonatal birthweight, but not to poorer clinical outcomes in the HIV-exposed children PMID:29323435
The -221G>C polymorphism of MBL2, the -159C>T polymorphism of CD14 and the TNF-857 polymorphism of TNF-a are risk factors for spinal spinal tuberculosis (TB) and may be involved in the development of spinal TB in the Chinese population. These factors are indicators of susceptibility to spinal TB and require clinical attention. PMID:29298876
genetic variation of CD14, rs5744455, is related to the susceptibility to laryngeal cancer, providing a theoretical basis for the study of the pathogenesis of laryngeal PMID:29077168
Calcitriol regulates immune genes CD14 and CD180 to modulate LPS responses in human trophoblasts. PMID:29089453
The findings suggest that smoking and the presence of TNFalpha-308 GA/AA genotypes may increase the risk for peri-implantitis, while CD14-159 polymorphic CT/TT genotypes decrease the risk. PMID:28906511
Our results demonstrate that the expression of CD163 and CD206 in monocytes is modulated by LPS in vitro; LPS induces CD163 expression and downregulates the spontaneously increased expression of CD206 PMID:25914252
may be a simple and easy approach to assess by RT-FCM the reaction between NO and superoxide ion in whole blood monocytes..The no-wash, no-lyse staining protocol with CD45-KO and CD14-PB allows to differentiate clearly and to gate in the monocyte population in near-physiological conditions PMID:25758468
Association of polymorphic markers of chemokine genes, their receptors, and CD14 gene with coronary atherosclerosis PMID:29369549
data confirm that engineered human cells expressing TLR4, MD2 and CD14 can respond to CMP with NF-kappaB activation and the response can be influenced by variations in CMP-mannosylation PMID:29281684
hypothesize that CD14(-159C/T) polymorphic variants might be one of genetic components in the response to attenuated M. bovis BCG bacilli PMID:29281719
Higher sCD14 levels were found in twins with schizophrenia or bipolar disorder compared to their not affected co-twins, and higher sCD14-levels were associated with psychotic symptoms. PMID:28039552
novel observation that sCD14 compared with lipopolysaccharide binding protein, offers a preferred target to ameliorate TLR especially TLR4-induced inflammation and insulin resistance in human obesity and metabolic syndrome PMID:26880233
For asthma, there was suggestive evidence of a gene-by-environment interaction for the CD14 variant rs2569190 (Interaction P = 0.16) but not for the TLR4 variants rs4986790 and rs4986791. PMID:27977294
the simultaneous detection of both plasma sCD14 and IL-6 is a promising diagnostic approach to identify active pulmonary tuberculosis, and further, measurement of TNF-alpha and sCD163 can identify the most severe cases of tuberculosis. PMID:27591510
the methylation levels in the promoter region of CD14 gene were higher in the Finnish compared to Russian Karelian children PMID:27434019
Anti-apoA-1 IgG are independent predictors of nonfatal incident coronary artery disease in the general population. The strength of this association is dependent on a functional polymorphism of the CD14 receptor gene, a finding suggesting a gene-autoantibody interaction for the development of CAD. PMID:29074586
CD14 haplotypes and sCD14 are important mediators of lung function among those with COPD in this occupationally-exposed population. PMID:28302109
IL-1beta-primed dendritic cells express high levels of CD14 that mediate IL-17 production through direct interaction with T cells. PMID:27550748
The distribution of the CD14 rs2569190 genotypes and allele frequencies showed significant differences between the test and control groups. Prevalence of the polymorphic TT genotype and T allele of the CD14 rs2569190 were significantly increased in GCP patients when compared with healthy controls PMID:28829191
Presepsin is significantly higher in preterm infants with early-onset sepsis compared with uninfected infants. PMID:27925621
Neovascularized atherosclerotic lesions selectively associate with lower blood levels of CD14+ and CD14(high)CD16- monocytes independently of systemic inflammatory activity. PMID:27751505
CXCR7 mediates CD14(+)CD16(+) monocyte transmigration across the blood brain barrier, and is a potential therapeutic target for neuro AIDS. PMID:28754798
META-ANALYSIS: association between the -159C/T polymorphism in the promoter region of the CD14 gene and sepsis PMID:28122493
In LPS-stimulated HEK293 cells with low CD14 and high TLR4, no accumulation of PI(4,5)P2 occurred. With an increasing amount of CD14 and decrease of TLR4, 2 peaks of PI(4,5)P2 appeared, approaching those found in LPS-stimulated cells expressing CD14 alone. This suggests that LPS-induced accumulation of PI(4,5)P2 that maximizes TLR4 signaling is controlled by CD14, whereas TLR4 can fine tune this via PI(4,5)P2 turnover. PMID:27418354
Intermediate CD14++CD16+monocytes might be closely related to the pathogenesis of atrial fibrillation and reflect functional remodelling of the left atrium. PMID:26826137
In 121 primary sclerosing cholangitis patients, the CD14 -260C>T genotype was associated with development of dominant bile duct strictures (P = 0.02). In 365 liver transplantation patients, TT carriers (4.1%) were protected against the formation of nonanastomotic biliary strictures versus CC/CT patients (12.6%; P = 0.01). PMID:26970220
response of hPdLSCs to bacterial LPS is strongly augmented by sCD14 PMID:27504628
analysis of plasma CXCL10, sCD163 and sCD14 in virological suppression and risk of cardiovascular disease PMID:27355513
TGM2 has a role in macrophage differentiation via mechanisms involving CD14 and SR-AI receptors. PMID:27378395
we found that higher percentages of circulating CD14+CD204+, CD14+CD163+CD204+ M2-like monocytes were significantly associated with TNM stage, lymph node metastasis, and histological differentiation. PMID:28639912
these results indicate that CD14 is a co-receptor of TLR4 in the S100A9-induced cytokine response. PMID:27228163
our data showed the contribution on the TLR4+896A/G and CD14-159C/T polymorphism-related immune dysfunction including increased non-classical (inflammatory) monocyte proportion-related LPS hyper-inflammatory response and decreased classical (phagocytic) monocyte proportion-related impaired phagocytosis in febrile acute de-compensated cirrhotic patients complicated with severe sepsis. PMID:27861595
Plasma presepsin concentrations are associated with the severity of sepsis and its outcome. PMID:28099244
Presepsin is a valuable new biomarker for defining severe infections in cirrhosis, proving same efficacy as procalcitonin. However, it is not a useful marker of short-term mortality. PMID:27895404
Patients with CD14 C (-159) T gene polymorphism, a co-receptor of TLR4, have an increased risk of NAFLD development. PMID:27895422
Single nucleotide polymorphism CD14-159 C/T was linked to greater Atopic dermatitis risk at 2 to 3 years of age. PMID:27274543
While there was no association with any respiratory phenotype (as determined by symptoms), the CD14 CT/TT genotype appeared to be protective to increased exposure to NO2 and NO PMID:27142357
CD14 expression is significantly upregulated in human masticatory mucosa during wound healing PMID:28005267
we report that exogenous CnB is taken up by cells in a time- and concentration-dependent manner via clathrin-dependent receptor-mediated internalization. Our findings further confirm that uptake is mediated by the TLR4/MD2 complex together with the co-receptor CD14 PMID:27090571
Cord blood sCD14 may be a useful biomarker for predicting infant wheeze and prolonged cough by 1 year of age. PMID:27144407
Presepsin is a valuable biomarker to detect patients with acute pyelonephritis PMID:27889429
diagnostic accuracy of presepsin (sCD14-ST) as a biomarker of infection and sepsis PMID:27823951

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Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor. Secreted. Membrane raft. Golgi apparatus.

Tissue Specificity

Detected on macrophages (at protein level). Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.

Database Links

HGNC: 1628

UNIGENE: Hs.163867

KEGG: hsa:929

STRING: 9606.ENSP00000304236

OMIM: 158120

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