Abbreviation
Recombinant Human IL16 protein, partial (Active)
Purity
>95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 1.0 pg/ml, corresponding to a specific activity of >1.0x109 IU/mg.
Alternative Names
HGNC:5980; HsT19289; IL-16; IL16; IL16_HUMAN; Interleukin 16 precursor; Interleukin-16; LCF; Lymphocyte chemoattractant factor; Neuronal interleukin 16; NIL16; prIL 16; PrIL16 ; Prointerleukin 16
Species
Homo sapiens (Human)
Expression Region
1203-1332aa
Target Protein Sequence
MPDLNSSTDS AASASAASDV SVESTAEATV CTVTLEKMSA GLGFSLEGGK GSLHGDKPLT INRIFKGAAS EQSETVQPGD EILQLGGTAM QGLTRFEAWN IIKALPDGPV TIVIRRKSLQ SKETTAAGDS
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
IL-16 functions as a natural ligand for CD4, driving chemoattraction of CD4+ T cells, monocytes, and eosinophils—making validated chemotactic activity essential for any in vitro study of this cytokine. This tag-free recombinant human IL-16 partial protein (1203–1332 aa) induces robust chemotaxis of human CD4+ T lymphocytes at concentrations between 1.0 and 100 ng/ml, providing a reliable positive control and functional reagent for migration assays, downstream CD4-mediated signaling studies involving p56lck and MAPK pathways, and antibody validation workflows such as ELISA standard curves or Western blot controls. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that can confound immune cell readouts, which supports use in sensitive primary cell chemotaxis assays and in vivo inflammation models where spurious innate immune activation must be excluded. Purity exceeding 95% by SDS-PAGE, combined with this stringent endotoxin control, satisfies the quality criteria typical for quantitative functional bioassays and reference standard applications.
