Abbreviation
Recombinant Human IL1B protein (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 1.0 pg/ml, corresponding to a specific activity of >1.0x109 IU/mg.
Alternative Names
Catabolin; H1; IFN beta inducing factor; IL 1; IL 1 beta; IL-1 beta; IL1; IL1 BETA; IL1B; IL1B_HUMAN; IL1F2; Interleukin 1 beta; Interleukin 1 beta precursor; interleukin 1; beta; Interleukin-1 beta; OAF; Osteoclast activating factor; OTTHUMP00000162031; Preinterleukin 1 beta; Preinterleukin beta; Pro interleukin 1 beta
Species
Homo sapiens (Human)
Expression Region
117-269aa
Target Protein Sequence
APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV VFSMSFVQGE ESNDKIP VAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQ FPNW YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
IL-1β is a master regulator of innate immunity and inflammation, driving NF-κB and MAPK signaling cascades that make precise functional reconstitution of this cytokine essential for mechanistic studies. This tag-free recombinant human IL-1β, encompassing the full mature sequence (residues 117–269), demonstrates exceptional potency with an ED50 below 1.0 pg/ml in murine D10S cell proliferation assays, corresponding to a specific activity exceeding 1.0 × 10⁹ IU/mg — providing a reliable reference standard for cytokine detection assays and a potent stimulus for cell-based activation, differentiation, and apoptosis experiments. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that commonly confound immune cell assays, making this preparation appropriate for NF-κB/MAPK pathway dissection, PBMC or primary T-cell functional studies, and in vivo inflammation models. Purity exceeding 97% by SDS-PAGE further supports use as a coating antigen or positive control in ELISA and antibody validation workflows.
