Recombinant Mouse Prolactin (Prl) (Active)

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Code: CSB-AP000071MO

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US$142

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Express system: E.Coli

Species: Mus musculus (Mouse)

Tag Info: Tag-Free

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Product Details

Abbreviation

Recombinant Mouse Prl protein (Active)

Purity

>98% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliteration assy using rat Nb2-11 cells is less than 1.0 ng/ml, corresponding to a speicfic activity of> 1.0 x10^6 IU/mg.

Uniprot NO.

P06879

Research Area

Signal Transduction

Alternative Names

Prl; Prolactin; PRL

Species

Mus musculus (Mouse)

Source

E.Coli

Expression Region

30-226aa

Target Protein Sequence

LPICSAGDCQ TSLRELFDRV VILSHYIHTL YTDMFIEFDK QYVQDREFMV KVINDCPTSS LATPEDKEQA LKVPPEVLLN LILSLVQSSS DPLFQLITGV GGIQEAPEYI LSRAKEIEEQ NKQLLEGVEK IISQAYPEAK GNGIYFVWSQ LPSLQGVDEE SKILSLRNTI RCLRRDSHKV DNFLKVLRCQ IAHQNNC
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

22.4 kDa

Protein Length

Full Length of Mature Protein

Tag Info

Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Buffer

0.2 μm filtered PBS, pH 7.4 ,lyophilized

Lead Time

5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Troubleshooting and FAQs

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Datasheet & COA

Please contact us to get it.

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Description

Prolactin drives JAK2/STAT5 signaling through the prolactin receptor, making validated bioactivity essential for meaningful downstream results. This tag-free recombinant mouse Prl, covering the full mature sequence (residues 30–226), demonstrates an ED50 below 1.0 ng/ml in Nb2-11 cell proliferation assays, corresponding to a specific activity exceeding 1.0 × 10⁶ IU/mg—performance that supports receptor binding and competition assays, STAT5 phosphorylation studies, and cell-based signaling readouts such as cAMP induction. Endotoxin levels below 1.0 EU/μg, combined with purity greater than 98% by SDS-PAGE, satisfy the criteria typically required for in vivo pharmacology models investigating lactation, metabolic regulation, or immune modulation, as well as for use as a quantitative standard in immunoassays and antibody development campaigns. The authentic mature sequence also provides a suitable basis for structure-function studies probing receptor-binding determinants.