Abbreviation
Recombinant Human PROK1 protein (Active)
Purity
>98% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using bovine EJG cells is less than 2.0 μg/ml, corresponding to a specific activity of >500 IU/mg.
Research Area
Signal Transduction
Alternative Names
Black mamba toxin related protein; EG VEGF; EG-VEGF; EGVEGF; Endocrine-gland-derived vascular endothelial growth factor; Mambakine; PK1; PRK1; PROK1; PROK1_HUMAN; Prokineticin 1; Prokineticin-1
Species
Homo sapiens (Human)
Expression Region
20-105aa
Target Protein Sequence
AVITGACERD VQCGAGTCCA ISLWLRGLRM CTPLGREGEE CHPGSHKVPF FRKRKHHTCP CLPNLLCSRF PDGRYRCSMD LKNINF
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4, 0.02 % Tween-20
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
Prokineticin-1 functions as a potent angiogenic mitogen structurally related to VEGF, making it a critical tool for dissecting endocrine-gland vascular biology and tumor angiogenesis signaling. This tag-free recombinant protein spans the full mature sequence (residues 20–105) and demonstrates robust bioactivity, with an ED50 below 2.0 μg/ml in bovine EJG cell proliferation assays corresponding to a specific activity exceeding 500 IU/mg — data that supports use in cell proliferation and survival assays, angiogenesis models, and receptor-ligand binding studies via ELISA or SPR. The E. coli expression system yields a non-glycosylated form that provides a well-defined baseline for experiments where glycosylation-independent PROKR1/PROKR2 signaling is under investigation. Purity exceeding 98% by SDS-PAGE combined with endotoxin levels below 1.0 EU/μg satisfies the criteria typical for sensitive in vivo tumor biology models and primary cell-based wound healing assays.
