Abbreviation
Recombinant Human HBEGF protein (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of >1.0x106 IU/mg.
Alternative Names
diphtheria toxin receptor (heparin-binding EGF-like growth factor); diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor); Diphtheria toxin receptor; DT R; DT-R; DTR; DTS; DTSF; HB-EGF; HBEGF; HBEGF_HUMAN; HEGFL; Heparin binding EGF like growth factor; Heparin binding epidermal growth factor; Heparin binding epidermal growth factor like growth factor; Heparin-binding EGF-like growth factor; Proheparin binding EGF like growth factor
Species
Homo sapiens (Human)
Expression Region
63-148aa
Target Protein Sequence
DLQEADLDLL RVTLSSKPQA LATPNKEEHG KRKKKGKGLG KKRDPCLRKY KDFCIHGECK YVKELRAPSC ICHPGYHGER CHGLSL
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered 20 mM PB, pH 7.4, 130 mM NaCl
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
Heparin-binding EGF-like growth factor serves as both a potent mitogen signaling through EGFR/ErbB receptors and the physiological receptor for diphtheria toxin, making it a critical tool in cancer biology and receptor-ligand interaction studies. This tag-free recombinant protein spans the full mature sequence (residues 63–148) and demonstrates exceptional potency, with an ED50 below 1 ng/mL in Balb/c 3T3 proliferation assays corresponding to a specific activity exceeding 1.0 × 10⁶ IU/mg — providing a reliable basis for cell proliferation and survival assays, wound healing models, and in vivo tumor biology studies. Produced in *E. coli*, the protein lacks glycosylation, which makes it particularly appropriate for receptor-ligand binding and competition assays via SPR or ELISA where uniform molecular species and precise molar quantification are essential. Purity exceeding 97% by SDS-PAGE combined with endotoxin levels below 1.0 EU/μg satisfies the criteria typical for cell-based functional assays and antibody characterization workflows.
