Abbreviation
Recombinant Human BTC protein, partial (Active)
Purity
>98% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.05 ng/ml, corresponding to a specific activity of >2.0x107 IU/mg.
Research Area
Signal Transduction
Alternative Names
BTCProbetacellulin [Cleaved into: Betacellulin; BTC)]
Species
Homo sapiens (Human)
Expression Region
32-111aa
Target Protein Sequence
DGNSTRSPET NGLLCGDPEE NCAATTTQSK RKGHFSRCPK QYKHYCIKGR CRFVVAEQTP SCVCDEGYIG ARCERVDLFY
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4, with 0.02 % Tween-20
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
Betacellulin is a potent EGF family ligand that activates EGFR/ErbB signaling cascades critical to pancreatic β-cell neogenesis, wound healing, and epithelial proliferation. This tag-free recombinant human BTC, spanning the mature processed region (residues 32–111), demonstrates exceptional receptor-binding potency with an ED50 below 0.05 ng/ml in Balb/c 3T3 proliferation assays, corresponding to a specific activity exceeding 2.0 × 10⁷ IU/mg — providing a reliable positive control and dose–response standard for cell proliferation assays, stem cell or β-cell differentiation studies, and EGFR/ErbB receptor-ligand binding experiments via SPR or competition ELISA. E. coli expression yields a non-glycosylated protein that eliminates glycan-related variability, enabling cleaner interpretation of receptor-binding kinetics and ligand competition data. Purity exceeding 98% by SDS-PAGE combined with endotoxin levels below 1.0 EU/μg satisfies the criteria typical for cell-based angiogenesis and wound healing models as well as in vivo tumor biology studies.
