Abbreviation
Recombinant Human NTF4 protein (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures is less than 50 ng/ml, corresponding to a specific activity of >2.0x104 IU/mg
Research Area
Neuroscience?
Alternative Names
GLC10; GLC1O; Neurotrophic factor 4; Neurotrophic factor 5; Neurotrophin 4; Neurotrophin 4/5; Neurotrophin 5 (neurotrophin 4/5); Neurotrophin 5; Neurotrophin-4; Neurotrophin-5; Neutrophic factor 4; Neutrophic factor 5; NT 4; NT 4/5; NT 5; NT-4; NT-5; NT4; NT4/5; NT4P; NT5; NTF4; NTF4_HUMAN; NTF5
Species
Homo sapiens (Human)
Expression Region
81-210aa
Target Protein Sequence
M+GVSETAPAS RRGELAVCDA VSGWVTDRRT AVDLRGREVE VLGEVPAAGG SPLRQYFFET RCKADNAEEG GPGAGGGGCR GVDRRHWVSE CKAKQSYVRA LTADAQGRVG WRWIRIDTAC VCTLLSRTGR A
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 5.5
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
Neurotrophin-4 signals through the TrkB receptor to promote neuronal survival, differentiation, and synaptic plasticity, making it a critical tool for investigating neurotrophic signaling pathways. This tag-free recombinant human NTF4 encompasses the full mature protein (residues 81–210) and demonstrates robust biological potency, with an ED50 below 50 ng/mL in a choline acetyltransferase induction assay using rat basal forebrain septal cultures, corresponding to a specific activity exceeding 2.0 × 10⁴ IU/mg. These validated activity data support use in neuronal survival and differentiation studies, TrkB receptor-ligand binding and competition assays via ELISA or SPR, and stem cell differentiation protocols requiring defined neurotrophic stimulation. Produced in *E. coli* — appropriate for NTF4, which is not natively glycosylated — this protein provides purity greater than 97% by SDS-PAGE alongside endotoxin levels below 1.0 EU/μg, satisfying the quality criteria typical for sensitive primary cell culture systems and *in vivo* neuroregeneration models.
