Abbreviation
Recombinant Human GDF7 protein (Active)
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
Less than 0.1 EU/ug as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 1.0 μg/ml, corresponding to a specific activity of > 1000 IU/mg.
Research Area
Signal Transduction
Alternative Names
bmp12; bone morphogenetic protein 12; GDF-7; Gdf7; GDF7_HUMAN; growth differentiation factor 7; Growth/differentiation factor 7
Species
Homo sapiens (Human)
Expression Region
322-450aa
Target Protein Sequence
TALAGTRTAQ GSGGGAGRGH GRRGRSRCSR KPLHVDFKEL GWDDWIIAPL DYEAYHCEGL CDFPLRSHLE PTNHAIIQTL LNSMAPDAAP ASCCVPARLS PISILYIDAA NNVVYKQYED MVVEACGCR
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
GDF7 (BMP12) plays a critical role in tendon and ligament differentiation, making it a key tool for studying musculoskeletal and connective tissue biology. This tag-free recombinant human GDF7, covering the full mature protein (residues 322–450), demonstrates an ED50 of less than 1.0 μg/ml in ATDC5 alkaline phosphatase induction assays, corresponding to a specific activity exceeding 1000 IU/mg—confirming robust BMP receptor engagement suitable for osteogenic and chondrogenic differentiation studies, receptor-ligand binding assays via SPR or ELISA, and in vivo regenerative medicine models. E. coli expression yields a non-glycosylated homodimer that provides a defined, consistent baseline for mechanistic signal transduction experiments without glycosylation-related variability. Purity exceeding 95% by SDS-PAGE combined with endotoxin levels below 0.1 EU/μg satisfies the criteria typical for cell-based differentiation assays and supports use as a reliable ELISA standard for antibody characterization workflows.
