Abbreviation
Recombinant Human FGF4 protein, partial (Active)
Purity
>96% as determined by SDSPAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF?receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of >2.0x106 IU/mg.
Alternative Names
FGF-4; Fgf4; FGF4_HUMAN; Fibroblast growth factor 4; fibroblast growth factor 4 splice isoform; HBGF-4; HBGF4; Heparin secretory-transforming protein 1; Heparin-binding growth factor 4; Hst; HST-1; HST1; HSTF-1; HSTF1; Human stomach cancer transforming factor from FGF related oncogene; K FGF; Kaposi Sarcoma Oncogene; KFGF; KS3; Oncogene HST; Transforming protein KS3
Species
Homo sapiens (Human)
Expression Region
25-206aa
Target Protein Sequence
GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4, 300 mM NaCl
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
FGF4 plays a critical role in embryonic development, angiogenesis, and tumor progression by signaling through FGFR1–FGFR4, making it a key target in both stem cell biology and cancer research. This tag-free recombinant human FGF4 (residues 25–206) encompasses the mature secreted form and demonstrates exceptional receptor-binding potency, with an ED50 below 0.5 ng/ml in a thymidine uptake assay using FGFR-transfected BaF3 cells, corresponding to a specific activity exceeding 2.0 × 10⁶ IU/mg — providing a reliable standard for cell proliferation assays, receptor-ligand binding studies via SPR or ELISA, and angiogenesis models. Produced in *E. coli*, the protein lacks glycosylation, which offers a defined, homogeneous preparation well suited for quantitative binding competition experiments where post-translational variability could confound results. Purity exceeding 96% by SDS-PAGE combined with endotoxin levels below 1.0 EU/μg satisfies the criteria typical for in vivo tumor biology models and stem cell differentiation studies.
