Abbreviation
Recombinant Human FGF10 protein, partial (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of >2.0x106 IU/mg.
Alternative Names
BB213776 ; fd11d03; FGF 10; FGF-10; FGF10; FGF10_HUMAN; Fibroblast growth factor 10; Keratinocyte growth factor 2; KGF 2; Produced by fibroblasts of urinary bladder lamina propria; wu:fd11d03; zgc:109774
Species
Homo sapiens (Human)
Expression Region
40-208aa
Target Protein Sequence
LGQDMVSPEA TNSSSSSFSS PSSAGRHVRS YNHLQGDVRW RKLFSFTKYF LKIEKNGKVS GTKKENCPYS ILEITSVEIG VVAVKAINSN YYLAMNKKGK LYGSKEFNND CKLKERIEEN GYNTYASFNW QHNGRQMYVA LNGKGAPRRG QKTRRKNTSA HFLPMVVHS
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered 2 × PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
FGF10 is a critical paracrine factor in epithelial-mesenchymal signaling, driving branching morphogenesis and tissue repair through FGFR2b activation — making receptor-binding potency essential for any in vitro application. This tag-free recombinant human FGF10 (residues 40–208, covering the mature signaling domain) demonstrates an ED50 below 0.5 ng/ml in a BaF3/FGFR proliferation assay, corresponding to a specific activity exceeding 2.0 × 10⁶ IU/mg, which supports use in cell proliferation assays, organoid differentiation protocols, and wound healing models where consistent mitogenic drive is required. The E. coli expression system produces a non-glycosylated protein that provides a suitable basis for receptor-ligand binding studies via SPR or ELISA, as well as for tumor biology experiments investigating FGF10-FGFR2b axis dysregulation in cancer. Purity exceeding 97% by SDS-PAGE combined with endotoxin levels below 1.0 EU/μg satisfies the criteria typical for cell-based functional assays and in vivo regenerative medicine studies.
