Abbreviation
Recombinant Human EPGN protein, partial (Active)
Purity
>98% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 300 ng/ml, corresponding to a specific activity of >3.3x103 IU/mg.
Alternative Names
ALGV3072; EPG; EPGN; EPGN_HUMAN; Epigen; Epigen precursor; Epithelial mitogen; PRO9904
Species
Homo sapiens (Human)
Expression Region
24-95aa
Target Protein Sequence
AVTVTPPITA QQADNIEGPI ALKFSHLCLE DHNSYCINGA CAFHHELEKA ICRCFTGYTG ERCEHLTLTS YA
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Partial of Isoform 2
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
Epigen is the most recently identified ligand of the EGF receptor and plays a distinctive role in epithelial proliferation and tumor biology, making it a valuable tool for dissecting EGFR signaling specificity. This tag-free recombinant human EPGN, spanning residues 24–95 of Isoform 2, encompasses the EGF-like domain required for receptor engagement and demonstrates confirmed mitogenic activity with an ED50 below 300 ng/mL in Balb/c 3T3 proliferation assays, corresponding to a specific activity exceeding 3.3 × 10³ IU/mg. The E. coli expression system yields a non-glycosylated protein that isolates the intrinsic receptor-binding potency of the EGF-like domain from glycan-mediated effects, providing a suitable basis for receptor-ligand binding studies via SPR or ELISA, cell proliferation and survival assays, and in vivo tumor biology models. Purity exceeding 98% by SDS-PAGE combined with endotoxin levels below 1.0 EU/μg satisfies the criteria typical for cell-based functional assays and antibody characterization workflows.
