Human Epigen(EPGN) ELISA kit

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Code: CSB-EL007719HU

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 1.56 ng/mL-100 ng/mL

Sensitivity: 0.39 ng/mL

Trial Size:

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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Alternative Names

ALGV3072 ELISA Kit; EPG ELISA Kit; EPGN ELISA Kit; EPGN_HUMAN ELISA Kit; Epigen ELISA Kit; Epigen precursor ELISA Kit; Epithelial mitogen ELISA Kit; PRO9904 ELISA Kit

Abbreviation

EPGN

Uniprot NO.

Q6UW88

Species

Homo sapiens (Human)

Sample Type

serum, plasma, tissue homogenates

Detection Range

1.56 ng/mL-100 ng/mL

Sensitivity

0.39 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human EPGN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	103
	Range %	96-107
1:2	Average %	102
	Range %	97-105
1:4	Average %	92
	Range %	85-96
1:8	Average %	97
	Range %	92-101

Recovery

The recovery of human EPGN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-103
EDTA plasma (n=4)	105	98-109

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
100	2.687	2.568	2.628	2.528
50	2.143	2.002	2.073	1.973
25	1.298	1.277	1.288	1.188
12.5	0.732	0.705	0.719	0.619
6.25	0.414	0.393	0.404	0.304
3.12	0.263	0.252	0.258	0.158
1.56	0.172	0.168	0.170	0.070
0	0.101	0.099	0.100

Troubleshooting and FAQs

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ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

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Human Epigen(EPGN) ELISA kit

Customer Reviews and Q&A

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■ Q&A

Are there any recombinant proteins in this kit?
If so, what are the host cells?
Are there any components containing azide, thimerosal or 2-ME?
What is the composition of the Stop solution?

Thanks for your inquiry!
Pls check our information:
Standard: CHO cell-derived recombinant protein
There is no component containing azide, thimerosal or 2-ME with this kit.
Stop solution: 4N sulfuric acid

Citations

Candidalysin activates innate epithelial immune responses via epidermal growth factor receptor Ho J,/,2019

Target Background

Function(From Uniprot)

Promotes the growth of epithelial cells. May stimulate the phosphorylation of EGFR and mitogen-activated protein kinases.

Gene References into Functions

Study shows how the EGFR ligands epiregulin (EREG) and epigen (EPGN) stabilize different dimeric conformations of the EGFR extracellular region. Results reveal how responses to different EGFR ligands are defined by receptor dimerization strength and signaling dynamics. These findings have broad implications for understanding receptor tyrosine kinase (RTK) signaling specificity. PMID:28988771
cloning of a related gene in mouse PMID:112783
EPG is an EGF-like growth factor and a low affinity ligand PMID:15611079

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Subcellular Location

[Isoform 1]: Membrane; Single-pass type I membrane protein.; [Isoform 2]: Membrane; Single-pass type I membrane protein.; [Isoform 3]: Secreted.; [Isoform 4]: Secreted.; [Isoform 5]: Secreted.; [Isoform 6]: Secreted.

Database Links

HGNC: 17470

UNIGENE: Hs.401237

KEGG: hsa:255324

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