Rat keratinocyte autocrine factor/Amphiregulin,KAF/AR ELISA Kit

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Rat keratinocyte autocrine factor/Amphiregulin,KAF/AR ELISA Kit

Name: Rat keratinocyte autocrine factor/Amphiregulin,KAF/AR ELISA Kit
Brand: CUSABIO
SKU: CSB-E07299r

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Code: CSB-E07299r

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 1.25 pg/mL-80 pg/mL

Sensitivity: 0.31 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Target Background

Target Name

amphiregulin

Alternative Names

Areg; Sdgf; Amphiregulin; AR; Schwannoma-derived growth factor; SDGF

Abbreviation

AREG

Uniprot NO.

P24338

Species

Rattus norvegicus (Rat)

Sample Type

serum, plasma, tissue homogenates

Detection Range

1.25 pg/mL-80 pg/mL

Sensitivity

0.31 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat KAF/AR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-98
1:2	Average %	95
	Range %	91-99
1:4	Average %	86
	Range %	80-92
1:8	Average %	96
	Range %	92-102

Recovery

The recovery of rat KAF/AR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	98-108
EDTA plasma (n=4)	97	92-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
80	2.538	2.616	2.577	2.413
40	1.939	2.059	1.999	1.835
20	1.364	1.386	1.375	1.211
10	0.882	0.870	0.876	0.712
5	0.549	0.541	0.545	0.381
2.5	0.341	0.356	0.349	0.185
1.25	0.253	0.258	0.256	0.092
0	0.168	0.160	0.164

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

The Rat Keratinocyte Autocrine Factor/Amphiregulin (KAF/AR) ELISA Kit is designed to measure Amphiregulin (AR) in rat serum, plasma, and tissue homogenates with exceptional accuracy and sensitivity.

With a detection range of 1.25 pg/mL-80 pg/mL and a sensitivity of 0.31 pg/mL, this quantitative assay can detect even the smallest amount of KAF/AR in your samples. The assay time is only 1-5 hours, and the required sample volume is just 50-100ul. The detection wavelength is 450 nm, ensuring reliable and precise results every time.

This kit is ideal for researchers investigating the role of KAF/AR in cancer、activates intracellular signaling, lung morphogenesis, breast bone tissue and oocyte maturation, and tissue integrity recovery after infection or injury（激活细胞内信号、肺形态发生及乳腺骨组织和卵母细胞的发育成熟、感染或损伤后的组织完整性恢复）. The sandwich measurement principle allows for accurate quantification of KAF/AR in your samples, making it a valuable tool for any cancer research project. With its quick assay time, low sample volume requirement, and precise results, this kit is a must-have for any researcher studying cancer.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.

Gene References into Functions

Report increased AREG transcript and protein levels in the lungs of rodents with DA-induced bronchiolitis obliterans. PMID:24816162
estrogen and progeterone increased cell proliferation in normal gland and mammary cancers through the increased production of amphiregulin PMID:21258428
may play a role in the ovulatory process of cycling rats as well as in the induction of ovulation in immature rats PMID:15291759
the expression of amphiregulin (AR), a member of the epidermal growth factor (EGF) family, is rapidly and highly up-regulated by PTH in several osteoblastic cell lines and bone tissues PMID:15509566
findings identify Amphiregulin as a factor for resistance of glioma cells to delta9-Tetrahydrocannabinol induced apoptosis PMID:19229996