Rat Kidney injury molecule 1,Kim-1 ELISA Kit

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Code: CSB-E08808r

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Size:

96T,5×96T,10×96T

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Sample Types: serum, plasma, urine

Detect Range: 0.313 ng/mL-20 ng/mL

Sensitivity: 0.228 ng/mL

Trial Size:

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Product Details
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Target Background

Product Details

Target Name

hepatitis A virus cellular receptor 1

Alternative Names

Havcr1; Kim1; Hepatitis A virus cellular receptor 1 homolog; HAVcr-1; Kidney injury molecule 1; KIM-1; T cell immunoglobulin and mucin domain-containing protein 1; TIMD-1; CD antigen CD365

Abbreviation

Kim-1

Uniprot NO.

O54947

Species

Rattus norvegicus (Rat)

Sample Type

serum, plasma, urine

Detection Range

0.313 ng/mL-20 ng/mL

Sensitivity

0.228 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of Rat Kim-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

CUSABIO's rat KIM-1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative determination of KIM-1 concentrations in serum, plasma, and urine. This assay exclusively recognizes rat KIM-1. The quantitative sandwich ELISA technique of this kit is based on KIM-1 antibody-KIM-1 antigen interactions and an HRP colorimetric detection system to detect the levels of KIM-1 in samples. The intensity of the color is positively proportional to the amount of bound KIM-1 in the initial step.

KIM-1 is a phosphatidylserine receptor that mediates the phagocytosis of apoptotic bodies and oxidized lipids. It is involved in HAV infections, autoimmunity, immune tolerance, and atopic diseases. In normal conditions, KIM-1 is expressed at low levels in the kidney and other organs, but its expression is remarkably increased following renal injury, especially after ischemia-reperfusion injury, enabling them to engulf apoptotic and necrotic cells during acute kidney injury (AKI). The urinary KIM-1 level is tightly linked to its tissue level and correspondingly related to kidney tissue damage. KIM-1 is a possible long-term renal outcome predictor in addition to being an early biomarker of AKI.

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Citations

Preservation of kidney function with combined inhibition of NADPH oxidase and angiotensin-converting enzyme in diabetic nephropathy Thallas-Bonke V. et al,/,2010
Camel milk attenuates methotrexate-induced kidney injury via activation of PI3K/Akt/eNOS signaling and intervention with oxidative aberrations Hany H. Arab.et al,/,2018
Inhibition of Autophagy Attenuated Ethylene Glycol Induced Crystals Deposition and Renal Injury in a Rat Model of Nephrolithiasis Liu Y.et al,/,2018
miR-182 enhances acute kidney injury by promoting apoptosis involving the targeting and regulation of TCF7L2/Wnt/B-catenins pathway HuicongLi.et al,/,2018
Wnt4 is significantly upregulated during the early phases of cisplatin-induced acute kidney injury Yi-Xin He.et al,/,2018
Protective effects of curcumin on diabetic nephropathy via attenuation of kidney injury molecule 1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL) expression and alleviation of oxidative stress in rats with type 1 diabetes Hassan Ghasemi, et al,/,2019
Chicoric acid prevents methotrexate-induced kidney injury by suppressing NF-κB/NLRP3 inflammasome activation and up-regulating Nrf2/ARE/HO-1 signaling Abd El-Twab SM,/,2019
Ferulic acid protects against methotrexate nephrotoxicity via activation of Nrf2/ARE/HO-1 signaling and PPARγ, and suppression of NF-κB/NLRP3 inflammasome axis Ayman M. Mahmoud,/,2019
Camel milk attenuates methotrexate-induced kidney injury: Via activation of PI3K/Akt/eNOS signaling and intervention with oxidative aberrations Arab HH,/,2019
Reno-protective effect of linagliptin against gentamycin nephrotoxicity in rats Mai M. Helmy, et al,/,2019
Formononetin Upregulates Nrf2/HO-1 Signaling and Prevents Oxidative Stress, Inflammation, and Kidney Injury in Methotrexate-Induced Rats Aladaileh S H, et al,/,2019
Pioglitazone attenuates kidney injury in an experimental model of gentamicin-induced nephrotoxicity in rats Medic B, et al,/,2019
Autophagy-endoplasmic reticulum stress inhibition mechanism of superoxide dismutase in the formation of calcium oxalate kidney stones Juening Kang, et al,/,2019
Impact of betanin against paracetamol and diclofenac induced hepato-renal damage in rats Motawi T K, et al,/,2019
The possible protective effects of saccharum officinarum l. (sugar cane) juice co-supplementation on gentamicin induced acute renal toxicity in adult albino rats Asmaa Y. A,/,2020
The effects of synthetic orally administrated insulin nanoparticles in comparison to injectable insulin on the renal function markers of type 1-diabetic rats N Keiripour,/,2020
Ameliorative Effect of Propolis Against Thioacetamide Induced Hepatorenal Injury in Adult Male Rats. Kidney Injury Molecule-1 (KIM-1) a Biomarker of Renal Injury FE SAMAR,/,2020
MiR-122-5p and miR ‐326‐3p promote cadmium‐induced NRK‐52E cell apoptosis by downregulating PLD1 W Yuan,/,2020
Circular RNA VMA21 ameliorates sepsis‐associated acute kidney injury by regulating miR‐9‐3p/SMG1/inflammation axis and oxidative stress Y Shi,/,2020
Role of ferroptosis induced by a high concentration of calcium oxalate in the formation and development of urolithiasis Z He,/,2020
HO-1/PINK1 Regulated Mitochondrial Fusion/Fission to Inhibit Pyroptosis and Attenuate Septic Acute Kidney Injury HB Li,/,2020
Novel biomarkers for post-contrast acute kidney injury identified from long non-coding RNA expression profiles G Chen,/,2021
Camel Milk Mitigates Cyclosporine-Induced Renal Damage in Rats: Targeting p38/ERK/JNK MAPKs, NF-κB, and Matrix Metalloproteinases HH Arab,/,2021
Involvement of NOX-4/JAK/STAT pathway in the protective effect of aprepitant against diclofenac-induced renal toxicity HM Hafez,/,2022
Cerium oxide nanoparticles attenuate the renal injury induced by cadmium chloride via improvement of the NBN and Nrf2 gene expressions in rats M Mostafa,/,2022
Renoprotective Effect of Vardenafil and Avanafil in Contrast-Induced Nephropathy: Emerging Evidence from an Animal Model IE Zisis,/,2022
Suppression of lncRNA GAS6-AS2 alleviates sepsis-related acute kidney injury through regulating the miR-136-5p/OXSR1 axis in vitro and in vivo H Cui,/,2022
Nephro‐protective effect of Daphnetin in hyperoxaluria‐induced rat renal injury via alterations of the gut microbiota R Zhou,/,2022
7-hydroxycoumarin modulates Nrf2/HO-1 and microRNA-34a/SIRT1 signaling and prevents cisplatin-induced oxidative stress, inflammation, and kidney injury in rats DH Sami,/,2022
Carvacrol mitigates vancomycin-induced nephrotoxicity via regulation of IkBα/p38MAPK and Keap1/Nrf2 signaling pathways: an experimental study with in silico evidence MM Khalaf,/,2022
Rosmarinic acid mitigates chlorpyrifos-induced oxidative stress, inflammation, and kidney injury in rats by modulating SIRT1 and Nrf2/HO-1 signaling MS Abduh,/,2022
Modulation Effects of Eugenol on Nephrotoxicity Triggered by Silver Nanoparticles in Adult Rats HR Aboelwafa,/,2022
Protective efficacy of Schizandrin B on ameliorating nephrolithiasis via regulating GSK3β/Nrf2 signaling-mediated ferroptosis in vivo and in vitro C Dong,/,2023
Effect of perinatal exposure to glyphosate and its mixture with 2, 4-D and dicamba on rat dam kidney and thyroid function and offspring's health AO Docea,/,2023
Oxalate induces the ossification of RTECs by activating the JAK2/STAT3 signaling pathway and participates in the formation of kidney stones Q Song,/,2023
Renogrit attenuates Vancomycin-induced nephrotoxicity in human renal spheroids and in Sprague-Dawley rats by regulating kidney injury biomarkers and creatinine/urea clearance A Balkrishna,/,2023
Wheat Grass Attenuates Acute Kidney Injury Secondary to Rhabdomyolysis in a Rat Model of Crush Syndrome A Balah,/,2023
Icariin Reduces Cadmium-induced Renal Injury in Rats J Zheng, X Yang, C Zhang, W Zhang, L Zeng,/,2024
Circ_001653 alleviates sepsis associated-acute kidney injury by recruiting BUD13 to regulate KEAP1/NRF2/HO-1 signaling pathway X Li, W Zhou, J Chen, L Zhou, Y Li, X Wu,/,2024
Nephroprotective Potential of Rhamnazin Against Cadmium-Induced Kidney Damages via Activation of Nrf2/Keap1 Pathway M Anjum, N Ehsan, A Tahir, M Batool,/,2025
Silymarin attenuates post-weaning bisphenol A-induced renal injury by suppressing ferroptosis and amyloidosis through Kim-1/Nrf2/HO-1 signaling modulation in male Wistar rats RE Akhigbe, AO Adekunle, MD Ajao,/,2025
Ergosterol alleviates lipopolysaccharide-induced acute kidney injury in rats via a mechanistic modulation of TLR4/p-p38/NF-κB, iNOS/NO and Nrf2/HO-1 signaling pathways FF Ali, DM Elroby Ali, ASM Kotb,/,2025
Serial Combination of Toxic and Ischemic Renal Damages Causes Subsequent Chronic, Irreversible, and Progressive Renal Disease in Rats GA Massaro, J Mercado-Hernández,/,2025

Target Background

Function(From Uniprot)

May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4. May play a role in kidney injury and repair.

Gene References into Functions

High KIM1 expression is associated with decreased proximal tubular epithelial cell function. PMID:29045706
urinary KIM1 may be a valuable biomarker for the early diagnosis of obstructive AKI, and the use of a colloidal gold immunochromatographic strip may be a promising method for the rapid detection of urinary KIM1. PMID:28075469
Results show that in contrast to other known molecules indicating renal injury and/or repair mechanisms, the chronic renal expression of Kim-1 is strictly confined to proximal cysts. PMID:27231899
blood biomarker that specifically reflects acute and chronic kidney injury PMID:24904085
Ischemic postconditioning can attenuate the renal ischemia reperfusion injury and downregulate the expression of Kim-1. PMID:22898836
These studies are the first direct demonstration of regulation of KIM-1 in response to oxalate exposure in renal epithelial cells in vitro and in vivo. PMID:22984472
The most sensitive, noninvasive biomarkers of HCBD-induced renal toxicity in Hanover Wistar rats were urinary alpha-GST and KIM-1. PMID:21905055
The Kim1 gene is regulated in an epigenetic fashion under conditions of nephrotoxicity. PMID:20660082
Results show Kim-1 outperforms SCr, BUN and urinary NAG in multiple rat models of kidney injury. PMID:20458318
KIM-1 as a sensitive molecular marker of different levels of tubular injury PMID:20305092
Urinary Kim-1 serve as a noninvasive, rapid, sensitive, reproducible method to detect early kidney injury in pathophysiological studies and in preclinical drug development studies for risk-benefit profiling of pharmaceutical agents. PMID:16174863
These results implicate involvement of kidney injury molecule 1 (Kim-1) in the pathogenesis of proteinuria-induced renal damage/repair. Urinary Kim-1 levels may serve as a marker of proteinuria-induced renal damage. PMID:16467126
Kim-1 is associated with development of renin-angiotensin system-mediated renal damage PMID:16896183
Urinary Kim-1 and kidney Kim-1/Havcr1 expression appear to be sensitive and tissue-specific biomarkers that will improve detection of early acute kidney injury PMID:17934191
Data demonstrate that injured kidney epithelial cells assumed attributes of endogenous phagocytes, and that KIM-1 was directly responsible for phagocytosis in cultured primary rat tubule epithelial cells and also porcine and canine epithelial cell lines. PMID:18414680
on longitudinal follow-up during antiproteinuric treatment increased renal Kim-1 expression is reversible in proportion to proteinuria reduction, likely reflecting reversibility of early tubular injury PMID:19225054
Cadmium-induced increase in Kim-1 expression occurs before the onset of necrosis and at a point where there is only a modest level of apoptosis in the proximal tubule. PMID:19371613
Kim-1 is a sensitive biomarker of the early stages of Cadmium-induced proximal tubule injury. PMID:19371616
Dipsticks can provide a sensitive and accurate detection of Havcr1. PMID:19387469
Kim-1 is a promising novel marker for the early, organ specific and noninvasive detection of brain death-induced donor kidney damage. PMID:19522876

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Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Families

Immunoglobulin superfamily, TIM family

Tissue Specificity

Expressed at a low level in normal kidney but are increased dramatically in postischemic kidney. Expressed in proliferating bromodeoxyuridine-positive and dedifferentiated vimentin-positive epithelial cells in regenerating proximal tubules.

Database Links

UNIGENE: Rn.11154

KEGG: rno:286934

STRING: 10116.ENSRNOP00000009573

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