Pig apolipoprotein A1 (Apo-A1) ELISA Kit

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Name: Pig apolipoprotein A1 (Apo-A1) ELISA Kit
Brand: CUSABIO
SKU: CSB-E06834p

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Code: CSB-E06834p

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 80 μg/mL-2000 μg/mL

Sensitivity: 50 μg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Target Background

Target Name

apolipoprotein A-I

Alternative Names

APOA1Apolipoprotein A-I ELISA Kit; Apo-AI ELISA Kit; ApoA-I ELISA Kit; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I ELISA Kit; ProapoA-I); Truncated apolipoprotein A-I] ELISA Kit

Abbreviation

APOA1

Uniprot NO.

P18648

Species

Sus scrofa (Pig)

Sample Type

serum, plasma, tissue homogenates

Detection Range

80 μg/mL-2000 μg/mL

Sensitivity

50 μg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Competitive

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig Apo-A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	89-103
1:2	Average %	90
	Range %	85-103
1:4	Average %	93
	Range %	88-100
1:8	Average %	89
	Range %	82-97

Recovery

The recovery of pig Apo-A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	96-104
EDTA plasma (n=4)	90	86-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μg/ml	OD1	OD2	Average
2000	0.342	0.348	0.345
800	0.533	0.483	0.508
480	0.682	0.657	0.670
200	1.021	0.987	1.004
80	1.267	1.325	1.296
0	1.903	1.954	1.929

Troubleshooting and FAQs

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ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This Pig APOA1 ELISA Kit was designed for the quantitative measurement of Pig APOA1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 80 μg/mL-2000 μg/mL and the sensitivity is 50 μg/mL.

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.

Gene References into Functions

Alterations in the Apo A-I pattern is a good indicator of the presence and severity of infectious diseases in the pig.Lower overall amounts of Apo A-I were observed in Salmonella typhimurium and Escherichia coli infections. PMID:25377528
blood level decreases during experimentally induced acute-phase processes in pigs PMID:15845530
down-regulation of apolipoprotein A-I and A-IV messages in the liver may be mediated by interleukin 6 and tumor necrosis factor-alpha PMID:15878672

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Subcellular Location

Secreted.

Protein Families

Apolipoprotein A1/A4/E family

Tissue Specificity

Major protein of plasma HDL, also found in chylomicrons. Synthesized predominantly in the intestine and the liver.

Database Links

UNIGENE: Ssc.807

KEGG: ssc:397691

STRING: 9823.ENSSSCP00000015994

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