Mouse Thyroid-Peroxidase,TPO ELISA Kit

Thyroid peroxidase (TPO) is a key enzyme in thyroid hormone biosynthesis, catalyzing the iodination of tyrosine residues in thyroglobulin and the coupling of iodotyrosines to form thyroxine (T4) and triiodothyronine (T3). TPO sits in the apical membrane of thyroid follicular cells and is required for normal thyroid function and hormone production. TPO serves as a major autoantigen in autoimmune thyroid diseases. Anti-TPO antibodies are important diagnostic markers for conditions such as Hashimoto's thyroiditis and Graves' disease.

The Mouse Thyroid-Peroxidase TPO ELISA Kit (CSB-E08353m) provides quantitative measurement of TPO in Mus musculus samples using a competitive assay principle. This kit works with serum, plasma, cell culture supernatants, and tissue homogenates. The detection range spans 2.5 ng/mL to 40 ng/mL with a sensitivity of 2.5 ng/mL. The assay requires 50-100 μL sample volume, measures absorbance at 450 nm wavelength, and can be completed within 1-5 hours.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in thyroid-related research to measure thyroid peroxidase levels in biological samples. Applications include autoimmune thyroid disease studies and biomarker research where thyroid peroxidase serves as a key analytical target.

• Autoimmune thyroid research: Detection of thyroid peroxidase in serum samples from mouse models to study Hashimoto's thyroiditis and related autoimmune conditions
• Thyroid biomarker studies: Quantification of thyroid peroxidase alongside other thyroid-related markers in comprehensive endocrine profiling
• Disease model characterization: Measurement of thyroid peroxidase levels in experimental animal studies examining thyroid dysfunction and autoimmune responses