Mouse Lysosomal Pro-X carboxypeptidase(PRCP) ELISA kit

Product Details

Customer Reviews and Q&A

Target Background

Home
Product
Kit
ELISA Kits
Mouse Lysosomal Pro-X carboxypeptidase(PRCP) ELISA kit

Name: Mouse Lysosomal Pro-X carboxypeptidase(PRCP) ELISA kit
Brand: CUSABIO
SKU: CSB-EL018636MO

Promotion

Code: CSB-EL018636MO

Rate （N/A）
Review （0）

Compare

Size:

96T,5×96T,10×96T

Pls inquire

Quantity:

Sample Types: serum, plasma, tissue homogenates, cell lysates

Detect Range: 37.5 pg/mL-2400 pg/mL

Sensitivity: 9.37 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

Interested in a trial size? Please leave a message below.

Send an Inquiry

Start an on-line Chat

Online ordering is currently available for U.S. customers only. For orders outside the U.S., please kindly submit an inquiry or start a chat with us.

Promotion

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Target Name

prolylcarboxypeptidase (angiotensinase C)

Alternative Names

Prcp ELISA Kit; Lysosomal Pro-X carboxypeptidase ELISA Kit; EC 3.4.16.2 ELISA Kit; Proline carboxypeptidase ELISA Kit; Prolylcarboxypeptidase ELISA Kit; PRCP ELISA Kit

Abbreviation

PRCP

Uniprot NO.

Q7TMR0

Species

Mus musculus (Mouse)

Sample Type

serum, plasma, tissue homogenates, cell lysates

Detection Range

37.5 pg/mL-2400 pg/mL

Sensitivity

9.37 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PRCP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-99
1:2	Average %	98
	Range %	92-103
1:4	Average %	93
	Range %	89-98
1:8	Average %	91
	Range %	87-96

Recovery

The recovery of mouse PRCP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-98
EDTA plasma (n=4)	94	93-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2400	2.542	2.520	2.531	2.377
1200	2.017	1.955	1.986	1.832
600	1.427	1.321	1.374	1.220
300	0.722	0.781	0.752	0.598
150	0.465	0.455	0.460	0.306
75	0.322	0.346	0.334	0.180
37.5	0.249	0.248	0.249	0.095
0	0.154	0.153	0.154

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This Mouse PRCP ELISA Kit was designed for the quantitative measurement of Mouse PRCP protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 37.5 pg/mL-2400 pg/mL and the sensitivity is 9.37 pg/mL.

■ Customer Reviews

Submit a Review here

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH.

Gene References into Functions

Prolylcarboxypeptidase (PRCP) converts AngII to Ang (1-7) but only at an acidic pH. Global PRCP deficiency causes heart and kidney alterations and a moderate rise in BP. PRCP is abundant in the kidney collecting tubules, where the prevailing pH is low. In collecting tubules, PRCP deficiency could result in impaired AngII degradation. Increased AngII at this nephron site stimulates Na reabsorption and increases BP. PMID:28160049
activity and expression in plasma and lung tissue stays unaffected in lung ischemia-reperfusion injury PMID:27437782
This study demonistrated that PRCP gene is broadly expressed in the brain. PMID:24161824
PRCP regulates cell growth, angiogenesis, and the response to vascular injury PMID:23744584
PRCP is an important regulator of energy and glucose homeostasis since its deletion significantly improves metabolic parameters in mice exposed to both standard chow diet and high-fat diet PMID:22454290
analysis of non-benzimidazole and brain-penetrant prolylcarboxypeptidase inhibitors PMID:22079761
This study showed that PRCP mutant mice have a significant decrease in fat mass, although an increase in lean mass was also observed; reduced leptin levels and increased energy expenditure were also found. PMID:22202165
Murine prolylcarboxypeptidase depletion induces vascular dysfunction with hypertension and faster arterial thrombosis. PMID:21297000
PRCP is an important component of melanocortin signaling and weight maintenance via control of active alpha-MSH1-13 levels. PMID:19620781