Mouse Lipopolysaccharide-binding protein(LBP) ELISA kit

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Mouse Lipopolysaccharide-binding protein(LBP) ELISA kit

Name: Mouse Lipopolysaccharide-binding protein(LBP) ELISA kit
Brand: CUSABIO
SKU: CSB-EL012775MO

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Code: CSB-EL012775MO

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Size:

96T,5×96T,10×96T

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Sample Types: serum, plasma, tissue homogenates

Detect Range: 12.5 ng/mL-800 ng/mL

Sensitivity: 3.12 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Alternative Names

LbpLipopolysaccharide-binding protein ELISA Kit; LBP ELISA Kit

Abbreviation

LBP

Uniprot NO.

Q61805

Species

Mus musculus (Mouse)

Sample Type

serum, plasma, tissue homogenates

Detection Range

12.5 ng/mL-800 ng/mL

Sensitivity

3.12 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse LBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-92
1:2	Average %	102
	Range %	97-107
1:4	Average %	98
	Range %	94-101
1:8	Average %	93
	Range %	90-96

Recovery

The recovery of mouse LBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93
EDTA plasma (n=4)	96	91-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
800	2.922	2.974	2.948	2.803
400	2.166	2.159	2.163	2.018
200	1.447	1.448	1.448	1.303
100	0.913	0.925	0.919	0.774
50	0.524	0.565	0.545	0.400
25	0.335	0.312	0.324	0.179
12.5	0.253	0.258	0.256	0.111
0	0.140	0.150	0.145

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Mouse Lipopolysaccharide-binding protein(LBP) ELISA kit

Description

Lipopolysaccharide-binding protein (LBP) is an acute-phase protein that functions in the innate immune response to bacterial infections. This glycoprotein binds to lipopolysaccharide from gram-negative bacteria and transfers it to CD14 and Toll-like receptor 4, which initiates inflammatory cascades. LBP levels rise significantly during sepsis and other inflammatory conditions. This makes it a valuable biomarker for monitoring immune activation and bacterial infection severity in research applications.

The Mouse Lipopolysaccharide-binding protein ELISA kit (CSB-EL012775MO) uses a quantitative sandwich measurement principle for detecting LBP in Mus musculus samples. This assay works with serum, plasma, and tissue homogenates with a detection range of 12.5 ng/mL to 800 ng/mL and sensitivity of 3.12 ng/mL. The protocol requires 50-100 μL sample volume and takes 1-5 hours to complete, with detection performed at 450 nm wavelength.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in research studies to measure lipopolysaccharide-binding protein levels in plasma samples from mouse models. Applications include inflammatory response research and studies examining how various interventions affect systemic biomarkers.

• Inflammatory biomarker profiling - Measurement of lipopolysaccharide-binding protein alongside other inflammatory markers including cytokines in plasma samples to assess systemic inflammatory responses
• Endocrine and metabolic research - Analysis of lipopolysaccharide-binding protein levels in studies investigating hormonal parameters and metabolic function in mouse models
• Intervention studies - Quantification of plasma lipopolysaccharide-binding protein to evaluate how experimental treatments or exposures affect inflammatory biomarker profiles

■ Customer Reviews

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Novel role of hesperidin improve obesity in HFD mice by modulating the composition of the gut microbiota Z Liu,/,2020
Gum arabic reduces inflammation, oxidative, and nitrosative stress in the gastrointestinal tract of mice with chronic kidney disease BH Ali,/,2020
Ameliorative Effect of Gum Acacia on Hookah Smoke-Induced Testicular Impairment in Mice BH Ali,/,2020
Comparative study of the anti-obesity and gut microbiota modulation effects of green tea phenolics and their oxidation products in high-fat-induced obese mice Z Liu,/,2021
Low potassium disrupt intestinal barrier and result in bacterial translocation H Wu,/,2022
Role of microbiota-gut-brain axis in natural ageing-related alterations in behavior Y Jing,/,2024
Hesperidin and Fecal Microbiota Transplantation Modulate the Composition of the Gut Microbiota and Reduce Obesity in High Fat Diet Mice T Liu, C Lei, Q Huang, W Song, C Li,/,2024
Gut symbiont-derived ursodeoxycholic acid promotes fatty acid oxidation to protect against renal ischemia-reperfusion injury M Xie, J Zheng, Y Yu, Q Yang, Z Zhou, J Xue,/,2025

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. Acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide.

Gene References into Functions

The LBP gene is a macrophage-specific LXR target that promotes foam cell survival and atherogenesis. PMID:24671012
LBP is a critical factor in the development of non-alcoholic fatty liver disease. PMID:27404046
LBP is a proinflammatory soluble adipokine that acts as a brake for adipogenesis, strengthening the negative effects of palmitate and LPS on adipocyte differentiation. PMID:26201685
Differential expression of LBP and TGFB1, along with other genes, in different mesenchymal stromal cells preparations, produces the variable responses to external stimuli. PMID:24664302
findings describe a novel role for LBP in normal hippocampal development and raise the possibility that some of the behavioral sequelae of early life stress are mediated by reduced expression of LBP during a critical period of neurodevelopment. PMID:21993208
The amino acid sequence for two mimic epitopes of the inflammatory site of LBP were determined to be WKAQKRFMKKSG and LKTRKLFWKYKD. PMID:20833181
LBP plays an important role in early alcohol-induced liver injury by enhancing LPS-induced signal transduction, most likely in Kupffer cells. PMID:11884468
LBP plays an important role in resistance to lethal Salmonella peritonitis (but not to oral or intravenous infection) by facilitating the role of neutrophils in mediating acute inflammation. PMID:12055258
role LBP plays in local pulmonary immune defenses to bacterial challenge PMID:12353926
In the case of intraperitoneal infection of LBP-deficient mice with Salmonella typhimurium, no local inflammatory response is evident, neutrophil influx is delayed, and the mice succumb to the infection. PMID:12370383
plays an essential role in the innate immune response to Gram-positive pneumococci PMID:12932360
Locally produced LBP is an essential component of an effective innate immune response to E. coli peritonitis. PMID:14638760
LBP is one of the critical molecules regulating the acute and chronic airway response to inhaled lipopolysaccharides (LPS). PMID:15356561
LBP knockout mice are protected from toxicity with a decrease in hepatic necrosis following acetaminophen challenge. This suggests a novel role for LBP in modulating acetaminophen-induced liver injury. PMID:15619225
LBP does not contribute to an effective host response in Mycobacterium tuberculosis infection in mice. PMID:15993364
LBP per se possesses a protective effect on acute necrotizing pancreatitis PMID:16163057
In conclusion, hemorrhagic shock and resuscitation to mice cause severe, LBP-mediated hepatocellular damage. PMID:16614372
Oxidized phospholipid inhibition of toll-like receptor (TLR) signaling is restricted to TLR2 and TLR4: roles for CD14, LPS-binding protein, and MD2 as targets for specificity of inhibition. PMID:18559343
LPS-binding protein mediates LPS-induced liver injury and mortality in the setting of biliary obstruction. PMID:18948440
Marked concomitant increases in plasma LBP and endotoxin-like activity following murine trypanosome infection might play an important role in the pathogenesis of trypanosomosis PMID:19022463
Paneth cells express LBP together with other proteins acting in the innate immune response of the gut, such as lysozyme, defensins and intelectin. PMID:19234712
Innate defense against lethal peritoneal infection with Salmonella requires the direct proinflammatory involvement of LPS-binding protein (LBP), a major nonredundant function of LBP in this model. PMID:11466385