Mouse Interleukin-19(IL19) ELISA kit

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Code: CSB-EL011612MO

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Size:

96T,5×96T,10×96T

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Sample Types: serum, plasma, tissue homogenates

Detect Range: 12.5 pg/mL-800 pg/mL

Sensitivity: 3.12 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Target Background

Product Details

Target Name

interleukin 19

Alternative Names

Il19Interleukin-19 ELISA Kit; IL-19 ELISA Kit

Abbreviation

IL19

Uniprot NO.

Q8CJ70

Species

Mus musculus (Mouse)

Sample Type

serum, plasma, tissue homogenates

Detection Range

12.5 pg/mL-800 pg/mL

Sensitivity

3.12 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL19 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	107
	Range %	96-112
1:2	Average %	103
	Range %	94-107
1:4	Average %	95
	Range %	86-99
1:8	Average %	100
	Range %	90-104

Recovery

The recovery of mouse IL19 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	88-106
EDTA plasma (n=4)	103	92-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	2.707	2.656	2.682	2.542
400	1.494	1.544	1.519	1.379
200	0.829	0.845	0.837	0.697
100	0.422	0.446	0.434	0.294
50	0.309	0.313	0.311	0.171
25	0.201	0.212	0.207	0.067
12.5	0.176	0.171	0.174	0.034
0	0.142	0.138	0.140

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Mouse Interleukin-19(IL19) ELISA kit

Description

This Mouse IL19 ELISA Kit was designed for the quantitative measurement of Mouse IL19 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL.

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Target Background

Function(From Uniprot)

May play some important roles in inflammatory responses. Up-regulates IL-6 and TNF-alpha and induces apoptosis.

Gene References into Functions

Data, including data from studies using knockout mice, suggest that interleukin-19 suppresses hapten-dependent skin inflammation in elicitation phase of contact hypersensitivity. PMID:29386478
Attenuated colitis in IL-19-deficient animals was associated with reduced numbers of IL-6-producing macrophages in the inflamed colonic lamina propria. Microbial-driven expression of IL-19 by intestinal macrophages may contribute to the pathogenesis of inflammatory bowel disease. PMID:28864472
IL-19 can promote angiogenesis in the absence of hypoxia by at least two distinct mechanisms: 1) direct effects on vascular cells and 2) indirect effects by stimulation of macrophages. PMID:27053520
IL-19 plays an anti-inflammatory role in the Th2-mediated colitis model. PMID:27942772
microglia may self-limit their inflammatory response by producing the negative regulator IL-19. PMID:25794104
Lack of IL-19 exacerbates intimal hyperplasia PMID:24814101
we delineate the detailed molecular pathway for IL-4 up-regulation of IL-19 in keratinocytes, which may play an important role in AD pathogenesis. PMID:24943510
Il-19 expression is increased in astrocytes following bacterial challenge. PMID:24677051
IL-19 is important for cutaneous wound healing because it upregulates KGF expression. PMID:23582717
IL-19 is a potent inhibitor of experimental atherosclerosis. PMID:23950143
IL-19 is pivotal in the pathogenesis of breast cancer. PMID:22186257
endogenous IL-19 is a constituent of the regulome that controls both processes in vivo PMID:22110701
IL-19 drives pathogenic innate immune responses in the colon. PMID:19834971
In a dust mite-induced murine model of asthma, the IL-19 level in asthmatic BALB/cJ mice is twice that of healthy control mice. PMID:15557163