This ELISA kit provides quantitative measurement of CXCL8 (IL-8) in monkey samples, supporting immunology research focused on neutrophil-mediated inflammation and chemokine signaling. Because CXCL8 levels can span orders of magnitude between baseline and acute inflammatory states, and because non-human primate models are critical for translational studies of sepsis, mucosal immunity, and vaccine-induced innate responses, this kit is designed to address those specific demands.
Detection Range: The 7.8–500 pg/mL range captures both constitutive CXCL8 secretion in resting tissues and the elevated concentrations seen during acute inflammation. Samples from LPS-challenged monkeys or inflamed tissue homogenates may require dilution, while unstimulated plasma typically falls within range without concentration.
Sensitivity: At 3.9 pg/mL, the kit detects basal CXCL8 in unstimulated cell culture supernatants and low-level circulating concentrations present before overt inflammatory onset, which is essential for kinetic studies of early innate immune activation.
Sample Compatibility: Validation across serum, plasma, cell culture supernatants, and tissue homogenates enables researchers to correlate systemic CXCL8 levels with local tissue production and in vitro stimulation assays within a single study using one standardized platform.
Assay Efficiency: The 1–5 hour protocol and 50–100 µL sample volume accommodate limited-volume NHP specimens and time-course designs requiring multiple sampling points from the same animal.
This kit is well suited for studies of primate sepsis models, respiratory infection-driven neutrophilia, and preclinical evaluation of anti-inflammatory therapeutics.