Human macrophage inflammatory protein 2,MIP-2 ELISA Kit

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Human macrophage inflammatory protein 2,MIP-2 ELISA Kit

Name: Human macrophage inflammatory protein 2,MIP-2 ELISA Kit
Brand: CUSABIO
SKU: CSB-E07420h

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Code: CSB-E07420h

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, cell culture supernates, urine

Detect Range: 3.12 pg/mL-200 pg/mL

Sensitivity: 7.81 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Alternative Names

C-X-C motif chemokine 2 ELISA Kit; Chemokine (C X C motif) ligand 2 ELISA Kit; Chemokine; CXC motif; ligand 2 ELISA Kit; CINC 2a ELISA Kit; CINC2a ELISA Kit; CINC3 ELISA Kit; CXC chemokine ELISA Kit; CXCL 2 ELISA Kit; Cxcl2 ELISA Kit; CXCL2_HUMAN ELISA Kit; Cytokine-induced neutrophil chemoattractant 3 ELISA Kit; GRO 2 ELISA Kit; GRO b ELISA Kit; GRO protein; beta ELISA Kit; Gro-beta ELISA Kit; GRO-beta(5-73) ELISA Kit; GRO-beta-T ELISA Kit; GRO2 ELISA Kit; GRO2 oncogene ELISA Kit; GROb ELISA Kit; GRObeta ELISA Kit; Growth regulated protein beta ELISA Kit; Growth-regulated protein beta ELISA Kit; GROX ELISA Kit; Hematopoietic synergistic factor ELISA Kit; HSF ELISA Kit; Macrophage inflammatory protein 2 alpha ELISA Kit; Macrophage inflammatory protein 2 ELISA Kit; Macrophage inflammatory protein 2-alpha ELISA Kit; Melanoma growth stimulatory activity beta ELISA Kit; MGSA b ELISA Kit; MGSA beta ELISA Kit; MIP 2 ELISA Kit; MIP 2a ELISA Kit; MIP2 alpha ELISA Kit; MIP2 ELISA Kit; MIP2-alpha ELISA Kit; MIP2A ELISA Kit; MIP2alpha ELISA Kit; SB-251353 ELISA Kit; SCYB 2 ELISA Kit; Scyb ELISA Kit; SCYB2 ELISA Kit; Small inducible cytokine subfamily B; member 2 ELISA Kit

Abbreviation

MIP-2

Uniprot NO.

P19875

Species

Homo sapiens (Human)

Sample Type

serum, plasma, cell culture supernates, urine

Detection Range

3.12 pg/mL-200 pg/mL

Sensitivity

7.81 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MIP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-100
1:2	Average %	97
	Range %	91-105
1:4	Average %	100
	Range %	95-106
1:8	Average %	93
	Range %	86-98

Recovery

The recovery of human MIP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	82	80-85
EDTA plasma (n=4)	89	85-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.352	2.452	2.402	2.276
100	1.812	1.819	1.816	1.690
50	1.174	1.273	1.224	1.098
25	0.703	0.723	0.713	0.587
12.5	0.425	0.428	0.427	0.301
6.25	0.301	0.321	0.311	0.185
3.125	0.201	0.214	0.208	0.082
0	0.125	0.127	0.126

Troubleshooting and FAQs

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Human macrophage inflammatory protein 2,MIP-2 ELISA Kit

Description

Macrophage inflammatory protein 2 (MIP-2) is a chemokine that plays an important role in inflammatory responses and immune cell recruitment. This protein belongs to the CXC chemokine family and functions as a potent chemoattractant for neutrophils, promoting their migration to sites of inflammation and tissue injury. Various cell types produce MIP-2, including macrophages, epithelial cells, and fibroblasts in response to inflammatory stimuli. This makes it an important mediator in both acute and chronic inflammatory processes.

The Human macrophage inflammatory protein 2,MIP-2 ELISA Kit (CSB-E07420h) is designed for quantitative measurement of MIP-2 in human samples. This sandwich ELISA can analyze serum, plasma, cell culture supernates, and urine with a detection range of 3.12 pg/mL to 200 pg/mL and sensitivity of 7.81 pg/mL. The assay requires 50-100 μL sample volume, measures at 450 nm wavelength, and can be completed within 1-5 hours.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

Researchers have used this ELISA kit to study inflammatory responses and chemokine signaling pathways in various disease contexts. Studies have applied the kit to quantify CXCL2 levels in biological samples as part of broader cytokine and chemokine profiling efforts. The kit supports research examining immune cell interactions and inflammatory mediator release in both clinical and experimental settings.

• Inflammatory disease research: Used to measure CXCL2 concentrations in serum samples from patients with community-acquired pneumonia compared to healthy controls as part of cytokine profiling studies
• Neuroinflammation studies: Applied to quantify CXCL2 levels in cerebrospinal fluid and cell culture supernatants alongside other inflammatory mediators in research examining central nervous system inflammation
• Cancer immunology research: Applied in chemokine release assays to measure CXCL2 concentrations in supernatants from natural killer cell and bladder cancer cell coculture experiments, supporting studies of immune cell-tumor interactions
• Chemokine profiling: Used as part of comprehensive chemokine panels to assess multiple inflammatory mediators simultaneously in transwell migration assays and cellular interaction studies

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity.

Gene References into Functions

The studies revealed that, although overall structural and oligomerization features of CXCL3 and CXCL2 are similar, prominent differences were observed in their surface characteristics, thus implicating a functional divergence. PMID:28928065
Taken together, the data of the present study demonstrated that TcpC can induce MIP2 production, which may contribute to the characteristic histological change associated with pyelonephritis. PMID:28765918
Functional effects data suggested that recombinant human CXCL2 significantly enhanced the migration, invasion ability of SMMC7721 hepatocellular carcinoma cells, and weakened adhesion ability. PMID:27117207
Reduced rate of sickle-related complications in Brazilian patients carrying HbF-promoting alleles at the BCL11A and HMIP-2 loci PMID:26888013
Results identify the CXCL2/MIF-CXCR2 axis as an important mediator in MDSC recruitment and as predictors in bladder cancer. PMID:27721403
Chronic inflammation contributes to the change of CXCL12 DNA methylation in buccal cells and that DNA methylation profile of CXCL12 promoter plays important role in development and progression of periodontal disease. PMID:27580404
high GRO-beta expression correlates with poor prognosis and contributes to ovarian cancer tumorigenesis and metastasis. PMID:26063953
In this review, a genetic variant in CXCL12 is described that is associated with type 2 diabetes mellitus and its complications. PMID:25085744
We have demonstrated that GRObeta, as an oncogene product, contributed to tumorigenesis and metastasis of HCC PMID:25801245
Our results demonstrated that resistance to anti-proliferative effects of CXCR2 may also arise from feedback increases in MIP-2 secretion. PMID:25682075
autophagy is required for Hepatitis B virus-induced NF-kappaB activation and release of IL-6, IL-8, and CXCL2 in Hepatocytes PMID:25708728
The results link CXCL1 and CXCL2 chemokines with bone marrow adiposity and implicate CXCR2 signaling in promoting effects of marrow fat on progression of skeletal tumors in bone. PMID:25802102
CXCL2 has antimicrobial activity against E. coli and S. aureus. PMID:12949249
Simultaneous targeting of hCAP-G2 and MIP-2A is a promising strategy for the development of antitumor drugs as a treatment for intractable tumours. PMID:24098805
our results demonstrate the diverse mechanisms by which CXCL2 and CXCL3 mediate normal and asthmatic airway smooth muscle cell migration PMID:23904157
CXCL12 and CXCR4 are related to formation of gastric tumors and lymph node metastasis PMID:21630055
Ubiquinol decreases monocytic expression and DNA methylation of the pro-inflammatory CXCL2 gene in humans. PMID:23021568
CXCL2, a WAT-produced chemokine being up-regulated in obesity, stimulates neutrophil adhesion to vis WAT endothelial cells. Activated neutrophils in obesity may influence vis WAT-ECs functions and contribute to WAT inflammation. PMID:23372021
This is the first report showing the role of CXCL2 in cancer-associated bone destruction. PMID:22771802
Anti-human ANXA1 antibodies and, to a lesser extent, anti-human ANXA4 antibodies increased MIP-2 or IL-8 production. PMID:22056994
Data suggest that GRObeta may function as an oncogene product and contribute to tumorigenesis and metastasis of esophageal squamous cell carcinoma. PMID:21677836
It can be hypothesized that for some targets, such as CXCL1 and CXCL2, additional signaling may be necessary to fully activate the 3'untranslated region-dependent human antigen R (HuR) function in airway epithelium PMID:21220697
A significantly increased expression of GRO-2, GRO-3, and IL-8 in colon carcinoma as compared to normal tissue, is reported. PMID:20162422
G-CSF stimulates the expression of the MIP-2 receptor via STAT3-dependent transcriptional activation of Il8rb PMID:20185584
modulation of the GRO beta concentration in the endometrium by inflammatory mediators may contribute to the normal and pathological processes of human reproduction by regulating the trafficking of neutrophils into the endometrium PMID:12892904
Neutrophil elastase, MIP-2, and TLR-4 have roles in progression of human sepsis and murine peritonitis PMID:15614130
CXCL2 tandem repeat promoter polymorphism is associated with susceptibiltiy to severe sepsis in the Spanish population. PMID:16421598
CXC chemokine CXCL10 and CC chemokine CCL2 serum levels increase with normal aging PMID:16697212
Inhibition of ERK phosphorylation decreased expression of Grob. PMID:17466952
data suggest that a tandem repeat polymorphism (AC)n at position -665 in the CXCL2 gene may be an independent predictor of mortality for severe sepsis PMID:17944017
Decrease of CXCL1 and -2 mediated by Curcumin is involved in the inhibition of metastasis in breast cancer cells. PMID:17999991
Peripheral neutrophilia and increased serum chemokines (IL-8 and MIP-2) may indicate hepatic injuries in glycogen storage disease type Ia. PMID:18191274
Resident tissue macrophages are the major source of MIP-2 and KC chemokines; these chemokines are newly synthesized products of signaling through Toll-like receptors. PMID:18322244
In colon epithelial cells, induction of MIP-2 alpha expression by tumor necrosis factor-alpha was accompanied by a concomitant reduction in miR-192 expression and miR-192 was observed to regulate the expression of MIP-2 alpha. PMID:18835392
Report gonadotropin-releasing hormone-regulated CXCL2 expression in human placentation. PMID:19369450