Human Transgelin-2(TAGLN2) ELISA kit

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Name: Human Transgelin-2(TAGLN2) ELISA kit
Brand: CUSABIO
SKU: CSB-EL023106HU

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Code: CSB-EL023106HU

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Citation （2）

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, cell lysates

Detect Range: 0.312 ng/mL-20 ng/mL

Sensitivity: 0.078 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Alternative Names

CDABP0035 ELISA Kit; epididymis tissue protein Li 7e ELISA Kit; HA1756 ELISA Kit; KIAA0120 ELISA Kit; SM22-alpha homolog ELISA Kit; TAGL2_HUMAN ELISA Kit; Tagln2 ELISA Kit; Transgelin-2 ELISA Kit

Abbreviation

TAGLN2

Uniprot NO.

P37802

Species

Homo sapiens (Human)

Sample Type

serum, plasma, cell lysates

Detection Range

0.312 ng/mL-20 ng/mL

Sensitivity

0.078 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TAGLN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-92
1:2	Average %	101
	Range %	98-103
1:4	Average %	92
	Range %	85-96
1:8	Average %	99
	Range %	93-102

Recovery

The recovery of human TAGLN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	92-98
EDTA plasma (n=4)	96	92-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.398	2.367	2.383	2.228
10	1.672	1.576	1.624	1.469
5	1.071	1.062	1.067	0.912
2.5	0.624	0.618	0.621	0.466
1.25	0.427	0.411	0.419	0.264
0.625	0.319	0.306	0.313	0.158
0.312	0.251	0.240	0.246	0.091
0	0.156	0.154	0.155

Troubleshooting and FAQs

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ELISA Data Analysis

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Description

This Human TAGLN2 ELISA Kit was designed for the quantitative measurement of Human TAGLN2 protein in serum, plasma, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Gene References into Functions

Study provides important evidence that hypoxia-inducible TAGLN2 is involved in the selection of cancer cells with enhanced EMT properties to overcome the detrimental environment of cancer cells as gamma radiation. PMID:30191639
Results show that Increased expression of TAGLN2 is associated with increasing tumor grade in glioma and poor patient survival. Knockdown experiments highlighted the function of TAGLN2 in promoting glioma cell invasion, the EMT phenotype, and tumor growth. PMID:29110682
Our data support a novel mechanism in diabetes-associated PDAC by which transgelin-2 mediates proliferation of PDAC cells upon insulin stimulation. PMID:28521289
Results suggest that TAGLN2 might regulate activation and migration of B-cells, in particular, the entry of activated B-cells into the follicle. PMID:28910360
we reviewed the basic characteristics and function of Transgelin-2 and its biological role in various types of diseases in order to provide the theoretical basis for further research and new perspectives on cancer development. PMID:28639888
Our data demonstrated that TAGLN2 might be an HBx induced positive host factor involved in HBV transcription and replication and HBx related liver fibrosis and tumorigenesis PMID:27402267
Transgelin-2 plays functional roles in the progression of cervical squamous cell carcinoma. Suppression of Transgelin-2 may be a new strategy for the treatment of cervical squamous cell carcinoma. PMID:26891454
It is a target gene of miR-1. PMID:26414725
Salvianolic acid A can reverse the paclitaxel resistance and inhibit the migration and invasion abilities of human breast cancer cells by down-regulating the expression of transgelin 2. PMID:26176734
Proteomics result showed TAGLN2 as the most significant overexpression in individual bladder cancer tissues and urine specimens, and thus represents a potential biomarker for noninvasive screening for bladder cancer. PMID:26081836
TAGLN2 modulates hypoxia-induced apoptosis via caspase-8 apoptotic pathway. Taken together, our data demonstrated the roles of miR-133a in hypoxia-induced apoptotic and implicate its potential in cardiac dysfunctions therapy. PMID:25421410
Transgelin-2, a sequence homolog of transgelin, whose role in the tumor development might be contradictory to the role of transgelin. PMID:24476357
PTTG1 and TAGLN2 are highly expressed in human pancreatic cancer, and the positive expression of PTTG1 is associated with the gender of cancer patients, suggesting that it may represent a potential therapeutic target for the treatment of pancreatic cancer PMID:23527717
our data propose a novel, oncogene-tumor suppressor interplay, where oncogenic PFTK1 confers HCC cell motility through inactivating the actin-binding motile suppressing function of TAGLN2 via phosphorylation. PMID:21577206
Data indicate that TAGLN2 may have an oncogenic function and may be regulated by miR-1, a tumor suppressive miRNA in HNSCC. PMID:21378409
in pulmonary adenocarcinoma, overexpression of TAGLN was strictly localized to the tumor-induced reactive myofibroblastic stromal tissue compartment, whereas overexpression of TAGLN2 was exclusively localized to the neoplastic glandular compartment PMID:19848416