Human Thrombospondin-4(THBS4) ELISA kit

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Name: Human Thrombospondin-4(THBS4) ELISA kit
Brand: CUSABIO
SKU: CSB-EL023490HU

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Code: CSB-EL023490HU

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Size:

96T,5×96T,10×96T

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Sample Types: serum, plasma

Detect Range: 7.8 ng/mL-500 ng/mL

Sensitivity: 1.95 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Alternative Names

Thbs4 ELISA Kit; thrombospondin 4 ELISA Kit; thrombospondin 4b ELISA Kit; Thrombospondin IV ELISA Kit; Thrombospondin-4 ELISA Kit; TSP4 ELISA Kit; TSP4_HUMAN ELISA Kit

Abbreviation

THBS4

Uniprot NO.

P35443

Species

Homo sapiens (Human)

Sample Type

serum, plasma

Detection Range

7.8 ng/mL-500 ng/mL

Sensitivity

1.95 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human THBS4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	89-97
1:2	Average %	101
	Range %	98-105
1:4	Average %	99
	Range %	95-104
1:8	Average %	92
	Range %	88-96

Recovery

The recovery of human THBS4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-99
EDTA plasma (n=4)	93	89-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
500	2.673	2.698	2.686	2.539
250	2.034	2.086	2.060	1.913
125	1.242	1.268	1.255	1.108
62.5	0.695	0.675	0.685	0.538
31.2	0.403	0.415	0.409	0.262
15.6	0.278	0.299	0.289	0.142
7.8	0.217	0.227	0.222	0.075
0	0.146	0.148	0.147

Troubleshooting and FAQs

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ELISA Data Analysis

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Description

Thrombospondin-4 (THBS4) is a matricellular glycoprotein belonging to the thrombospondin family, which plays important roles in extracellular matrix organization and cell-matrix interactions. This protein participates in various biological processes including angiogenesis, wound healing, and tissue remodeling. THBS4 expression occurs in multiple tissues and is particularly relevant in cardiovascular research, cancer biology, and inflammatory conditions. As a secreted protein, THBS4 can be detected in biological fluids. This makes it a valuable biomarker for research applications studying matrix remodeling and related pathological processes.

The Human Thrombospondin-4 ELISA kit (CSB-EL023490HU) is designed for quantitative measurement of THBS4 in human serum and plasma samples. This sandwich-based assay operates with a detection range of 7.8 ng/mL to 500 ng/mL and shows a sensitivity of 1.95 ng/mL. The assay requires 50-100 μL sample volume and can be completed within 1-5 hours. Detection occurs at 450 nm wavelength, providing researchers with a reliable tool for THBS4 quantification in Homo sapiens samples.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in clinical research to measure circulating thrombospondin 4 levels in human biological samples. The applications span cardiovascular disease research and neurological studies, with researchers using the kit to quantify this biomarker in plasma and cerebrospinal fluid samples from patient populations.

• Cardiovascular disease research: Studies of thrombospondin 4 levels in patients with congenital heart disease and pulmonary arterial hypertension, comparing biomarker concentrations across different disease severity groups
• Neurological studies: Measurement of thrombospondin 4 concentrations in cerebrospinal fluid samples as part of biomarker profiling panels
• Clinical biomarker studies: Quantification of circulating thrombospondin 4 in serum samples from fasted patients for biomarker correlation studies
• Patient stratification research: Analysis of plasma biomarker levels to distinguish between different clinical presentations and disease severities

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Thrombospondin-4 increases with the severity of peripheral arterial disease and is associated with diabetes Zierfuss B, et al,/,2019
Single‐Cell RNA Sequencing and Spatial Transcriptomics Reveal Pathogenesis of Meningeal Lymphatic Dysfunction after Experimental Subarachnoid Hemorrhage X Wang,/,2023
The role and mechanism of thrombospondin-4 in pulmonary arterial hypertension associated with congenital heart disease H Zeng, B Lan, B Li, H Xie, E Zhao, X Liu, X Xue,/,2024

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions and is involved in various processes including cellular proliferation, migration, adhesion and attachment, inflammatory response to CNS injury, regulation of vascular inflammation and adaptive responses of the heart to pressure overload and in myocardial function and remodeling. Binds to structural extracellular matrix (ECM) proteins and modulates the ECM in response to tissue damage, contributing to cardioprotective and adaptive ECM remodeling. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors and protects myocardium from pressure overload. May contribute to spinal presynaptic hypersensitivity and neuropathic pain states after peripheral nerve injury. May play a role in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury in a NOTCH1-dependent manner.

Gene References into Functions

establish a novel molecular mechanism by which miR-148a-3p upregulates Tsp-4 expression in tenocytes to promote angiogenesis by targeting KLF6, which could be helpful for the treatment of tendinopathy in the future PMID:29807011
Findings indicate that loss of miR-142 results in the over-expression of THBS4, which enhances HCC migration and vascular invasion. PMID:28177895
the present study demonstrates that THBS4 and lncRNA-THBS4-003 serve a significant role in PCa proliferation and migration via the MMP-9 and p38 MAPK signaling pathway. PMID:27357608
The THBS4 A387P polymorphism was associated with coronary artery disease in American population. PMID:25976449
Tsp4 functions as an ECM scaffold at myotendinous junctions, with potential therapeutic uses in tendon strengthening and repair PMID:24941943
THBS4 expression in breast cancer-associated extracellular matrix contributes to the activated stromal response exhibited during tumour progression PMID:23942617
the balancing selection target in THBS4 is likely represented by one or more variants that regulate tissue-specific and sex-specific gene expression PMID:23420636
The results of thid study suggested that injury-induced spinal TSP4 may contribute to spinal presynaptic hypersensitivity and neuropathic pain states. PMID:22745497
genetic polymorphism predicts cardiovascular risk in postinfarction patients with high HDL cholesterol and C-reactive protein levels PMID:22011848
Identify THBS4 as a powerful marker for diffuse-type gastric adenocarcinomas and to provide an initial characterization of its expression in the course of this disease. PMID:21701537
High THBS4 methylation resulting in inactivation of the gene is associated with colorectal cancer. PMID:20846368
thrombospondin-4 C-terminal peptide has a role in stimulating erythroid cell proliferation PMID:15474480
Promoter hypermethylation of thrombospondin 4 is associated with cutaneous T-cell lymphoma PMID:15897551
Homozygosity for thrombospondin-4 1186C (Arg387Pro) variant is a risk factor for myocardial infarction especially in older women. PMID:16923428
The functional analysis of the rat counterpart. PMID:7490284