Human Spondin-1(SPON1) ELISA kit

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Code: CSB-EL022599HU-IS

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 0.312ng/mL-20ng/mL

Sensitivity: 0.156ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Target Background

Product Details

Alternative Names

SPON1 ELISA kit; KIAA0762 ELISA kit; VSGPSpondin-1 ELISA kit; F-spondin ELISA kit; Vascular smooth muscle cell growth-promoting factor ELISA kit

Abbreviation

SPON1

Uniprot NO.

Q9HCB6

Species

Homo sapiens (Human)

Sample Type

serum, plasma, tissue homogenates

Detection Range

0.312ng/mL-20ng/mL

Sensitivity

0.156ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Neuroscience

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This ELISA kit provides quantitative measurement of Spondin-1 (SPON1) in human samples, supporting neuroscience research into this extracellular matrix glycoprotein involved in axon guidance, neuronal development, and emerging roles in Alzheimer's disease pathology. Because SPON1 functions at the intersection of amyloid precursor protein processing and neurodevelopmental signaling, researchers need precise quantification across both circulating and tissue-level compartments.

Detection Range: The 0.312–20 ng/mL range aligns with reported SPON1 concentrations in human plasma and tissue lysates, covering both baseline physiological levels and the elevated expression observed in neurodegenerative contexts. Most serum and plasma samples can be assayed neat or with minimal dilution, simplifying workflow planning.

Sensitivity: At 0.156 ng/mL, the kit detects SPON1 in low-abundance conditions such as cerebrospinal fluid-adjacent fractions, conditioned media from primary neuronal cultures, or samples from early-stage neurodegeneration where subtle expression changes carry biological significance.

Sample Compatibility: Validation across serum, plasma, and tissue homogenates enables researchers to correlate circulating SPON1 levels with localized expression in brain tissue within the same study, bridging biomarker discovery and mechanistic investigation.

Assay Efficiency: The 1–5 hour protocol and 50–100 µL sample volume accommodate limited-volume specimens typical of paired CSF-plasma biobanking studies and primary neuron culture experiments.

This kit is directly applicable to Alzheimer's disease biomarker research, axon guidance pathway studies, and neurodevelopmental disorder investigations.

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Target Background

Function(From Uniprot)

Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS. Major factor for vascular smooth muscle cell.

Gene References into Functions

gene expression experiment revealed that CYP2B6, SPON1, and GSG1L can be activated concomitantly through a constitutive androstane receptor (CAR) activation pathway PMID:27010727
SPON1 promotes metastatic progression through Fak and Src dependent pathway in human osteosarcoma. PMID:26032498
These data suggest that SPON1 may be associated with the differential rate of cognitive decline in Alzheimer disease. PMID:23535033
High SPON1 expression is associated with high-grade gliomas. PMID:24158112
Genome-wide scan of healthy human connectome discovers SPON1 gene variant influencing dementia severity PMID:23471985
downregulates osteoclast recruitment to the root side of periodontal tissue via low-density lipoprotein receptor-related protein 8 and inhibits differentiation of osteoclastic precursors PMID:21488757
These findings indicate that F-spondin regulates the differentiation of human cementoblast-like cells via BMP7 expression. PMID:22244873
The unique feature of F-spondin FS domain is the presence of three disulfide bonds associated with the N- and C-termini of the domain and a highly conserved N-linked glycosylation site. PMID:21569239
expression profile of VSGP/F-spondin identifies this molecule as a potential diagnostic marker or target for developing therapeutic strategies to treat ovarian carcinoma PMID:16103746
identified F-spondin as a promoting factor for cementoblastic differentiation PMID:16965763
The crystal structure of the reelin-N domain resolved to 2.0 A reveals a variant immunoglobulin-like fold and potential heparin-binding sites. PMID:18602404
These studies identify F-spondin as a novel protein in osteoarthritis cartilage, where it may act in situ at lesional areas to activate latent TGF-beta and induce cartilage degradation. PMID:18780763

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Subcellular Location

Secreted, extracellular space, extracellular matrix.

Tissue Specificity

Highest expression in lung, lower expression in brain, heart, kidney, liver and testis, and lowest expression in pancreas, skeletal muscle and ovary. Not expressed in spleen.

Database Links

HGNC: 11252

UNIGENE: Hs.643864

KEGG: hsa:10418

OMIM: 604989

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